Abstract:

Objective To evaluate rabbit renal ischemia reperfusion injury (IRI) by gray-scale contrast-enhanced ultrasonography (CEUS) and quantitative analysis techniques. Methods Ten New Zealand rabbits were performed left renal ultrasonography before surgery and were served as controls (R0 group, n=10), and then were randomly divided into R1 group (reperfusion for 30 min after ischemia for 30 min group, n=5) and R2 group (reperfusion for 60 min after ischemia for 30 min group, n=5) to establish the models of renal IRI. The pattern of renal perfusion was observed by CEUS, and time to peak (TP), amplitude of peak intensity(A), rising slope (β) of Qontraxt time-intensity curve and area under the curve (AUC) were analysed. Results With the increase of reperfusion injury, there was no significant change in renal cortex echo revealed by CEUS. TP and AUC increased, β values decreased (P<0.05 or P<0.01), and the change of AUC was even more significant (P<0.01). Besides, there was no significant change in A values among groups (P>0.05). Conclusion AUC is an effective index for evaluating rabbit renal IRI.

Key words: renal, ischemia reperfusion injury, gray-scale contrast-enhanced ultrasonography, quantitative analysis, rabbit