›› 2010, Vol. 30 ›› Issue (6): 656-.

• Original article (Basic research) • Previous Articles     Next Articles

Effects of different levels of short-term intermittent hypobaric hypoxia on antioxidants of blood and cardiovascular tissues in rats

YANG Wen-Jun1,2, ZHONG Mei-fang3, ZHANG Ning1, CHEN Fang-Li1, WU Xiu-feng2, ZHOU Zhao-nian2, CHEN Hong1   

  1. 1.Department of Pharmacology, Basic Medical College, Shanghai Jiaotong University, Shanghai 200025, China；2.Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China；3.Shanghai Institute of Health Sciences, Shanghai 201318, China
  • Online:2010-06-25 Published:2010-06-28
  • Supported by:

    National Basic Research Program of China, “973” Program, 2006CB504100；National Natural Science Foundation of China, 2009CB521907

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Abstract:

Objective To investigate the effects of different levels of short-term intermittent hypobaric hypoxia (IHH) on the antioxidant activity of blood and cardiovascular tissues in rats. Methods A total of 30 SD rats were assigned to control group, IHH 3 500 m group and IHH 5 000 m group (n=10 in each group). Rats in IHH 3 500 m group and IHH 5 000 m group were treated with IHH mimicking 3 500 m and 5 000 m altitude in a hypobaric chamber for a consecutive of 8 d, with 4 h each day. Rats of control group were treated under the same condition except for hypoxia intervention. After IHH treatment, the activity of superoxide dismutase (SOD) and catalase (CAT) and contents of malondialdehyde (MDA) in plasma, myocardium and aorta were measured, and the contents of plasma uric acid were detected. Results Compared with control group, the activity of plasma CuZn-SOD and myocardial total SOD and Mn-SOD increased in IHH 5 000 m group, while that of plasma Mn-SOD decreased (P<0.05). The activity of CAT and contents of MDA in plasma and myocardium increased in IHH 5 000 m group, and the contents of plasma uric acid increased as well (P<0.05). However, there was no significant difference in the activity of SOD and CAT and contents of MDA in aorta between IHH 5 0 00 m group and control group (P>0.05). The activity of plasma Mn-SOD in IHH 3 500 m group was significantly higher than that in control group (P<0.05), while there was no significant difference in the contents of plasma uric acid, activity of CAT and contents of MDA in myocardium and aorta between these two groups (P>0.05). Conclusion Moderate level of short-term IHH may enhance the endogenous antioxidants activity in plasma and myocardium in rats.

Key words: intermittent hypobaric hypoxia, blood, myocardium, aorta, antioxidants