›› 2010, Vol. 30 ›› Issue (12): 1530-.doi: 10.3969/j.issn.1674-8115.2010.12.020

• Original article (Clinical research) • Previous Articles     Next Articles

Effects of serum of patients with maintenance hemodialysis and dialysates on apoptosis of adipocytes

CAI Ling-ling1, GUO Li-li2, ZHU Xiao-juan1, GAO Feng-hou3, TAN Long-yi4, PAN Yu1, JIN Hui-min1   

  1. 1.Department of Nephrology, The Third People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 201900, China；2.Hemodialysis Unit, Baoshan Branch, The First People's Hospital, Shanghai Jiaotong University, Shanghai 200940, China；3.Experimental Center, The Third People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 201900, China；4.Clinical Laboratory, Baoshan Branch, The First People's Hospital, Shanghai Jiaotong University, Shanghai 200940, China
  • Online:2010-12-25 Published:2010-12-31
  • Supported by:

    Shanghai Municipal Health Bureau Foundation, 2007129

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Abstract:

Objective To investigate the effects of serum of patients with maintenance hemodialysis (HD), conventional dialysate (CD) and high purity dialysate (HPD) on apoptosis of adipocytes. Methods Blood samples of 20 patients with HD (ten were treated with CD and ten were treated with HPD) were collected, and serum was isolated for detection of tumor necrosis factor-α (TNF-α) by ELISA. Mature 3T3-L1 adipocytes induced in vitro were treated with hemodialysis patient serum (HDPS group), HDPS combined with CD (HDPS+CD group) and HDPS combined with HPD (HDPS+HPD group), respectively, and cells were harvested 48 h later. Morphological changes were observed by Hoechst33258 fluorescence staining, and cell apoptosis was detected by Annexin-V-FITC/PI double staining. Bacterial DNA from CD and HPD was assayed by PCR. Results It was revealed by ELISA that the serum level of TNF-α from patients with HD treated by CD was significantly higher than that from patients with HD treated by HPD (P<0.05). Typical apoptotic morphology changes were observed by fluorescence microscopy in HDPS group and HDPS+CD group, and the apoptosis rates in these two groups were significantly higher than that in HDPS+HPD group (P<0.05). PCR revealed that there were significant differences between the expression of bacterial DNA in CD and that in HPD (902.79±60.57 vs 454.87±32.22)(P<0.05). Conclusion HDPS may induce apoptosis of 3T3-L1 adipocytes, which may be related to the increased serum level of TNF-α. Compared with intervention by HDPS and CD, intervention by HDPS and HPD may yield lower apoptosis rate of adipocytes, and there may be lower expression of bacterial DNA in HPD.

Key words: 3T3-L1 adipocytes, apoptosis, tumor necrosis factor-α, conventional dialysate, high purity dialysate, bacterial DNA