›› 2011, Vol. 31 ›› Issue (7): 875-.doi: 10.3969/j.issn.1674-8115.2011.07.002

• Original article (Basic research) • Previous Articles     Next Articles

Association of Mycobacterium tuberculosis Rv1272 and Rv1273 with drug resistance

CHENG Xu-hong1,2, SUN Qing1, YAO Yu-feng2, ZHOU Ai-ping2,3, ZHAO Ying-wei1   

  1. 1.School of Biology and Basic Medical Sciences, Suzhou University, Suzhou 215123, China；2.Department of Medical Microbiology and Parasitology, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China；3.Tibet University for Nationalities, Xianyang 712082, China
  • Online:2011-07-28 Published:2011-07-27
  • Supported by:

    National Key Basic Research Development Program, “973” Program, 2009CB522605

PDF (PC)

1802

Abstract:

Objective To investigate the association of two proteins coded by Mycobacterium tuberculosis Rv1272 and Rv1273 with drug efflux. Methods The structures of Rv1272 gene, Rv1273 gene and proteins coded by them were analyzed by bioinformatics, and these two genes were amplified by PCR and cloned into expression vector pMF406 to generate the recombinant plasmids. After verification by sequence analysis, the recombinant plasmids were transformed into M.Smegmatis mc2155 by electroporation. The subcellular localization of these two proteins was probed by Western blotting, and the minimal inhibitory concentrations (MIC) of nine commonly used antibiotics drugs were determined by test tube method, with M. Smegmatis as control strain. Results It was verified by sequence analysis that the recombinant plasmids were successfully constructed, and it was identified by Western blotting that Rv1272 and Rv1273 were membrane proteins. It was revealed by test tube method that there was no significant difference in MIC of nine commonly used antituberculosis drugs between recombinant strains and control strain (P>0.05). Conclusion Rv1272 and Rv1273 are membrane proteins, and their relationship with drug resistance to Mycobacterium tuberculosis is uncertain.

Key words: Mycobacterium tuberculosis, Rv1272, Rv1273, membrane protein, minimal inhibitory concentration