›› 2011, Vol. 31 ›› Issue (12): 1661-.doi: 10.3969/j.issn.1674-8115.2011.12.001

• Original article (Basic research) •     Next Articles

Regulation of expression of osteoblast transcription factor SATB2 by BMP2 in osteoblastic differentiation of MSCs

ZUO Chi-jian1, LA Ting1, ZHANG Ning1, DAI Ke-rong1,2, ZHANG Xiao-ling1,2   

  1. 1.Laboratory of Orthopedic Cellular and Molecular Biology, Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences &|Shanghai Jiaotong University School of Medicine, Shanghai 200025, China；2.Shanghai Key Laboratory of Orthopaedic Implant, Department of Orthopaedics, the Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, China
  • Online:2011-12-28 Published:2012-01-04
  • Supported by:

    National Natural Science Foundation of China, 30871435；Shanghai Science and Technology Committee Foundation, 09540703600；Shanghai Education Committee Foundation, 10SG22, J50206

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Abstract:

Objective To investigate the role of bone morphogenetic protein 2 (BMP2) in the regulation of expression of osteoblast transcription factor SATB2 in osteoblastic differentiation of mesenchymal stem cells (MSCs). Methods Mesenchymal cell line C2C12 was stimulated by adenovirus-derived BMP2 (Adv-BMP2) for osteoblastic differentiation, and model of osteoblastic differentiation of C2C12 cells was established and verified. Real-Time PCR and Western blotting were employed to detect the expression of SATB2 mRNA and SATB2 protein respectively in osteoblastic differentiation treated by Adv-BMP2 for different time and by Adv-BMP2 of different concentrations. C2Cl2 cells treated with Adv-β-Gal were served as controls. Results After treatment by 150 pfu/cell Adv-BMP2 for 5 d, the expression of collagen type Ⅰ, bone sialoprotein and osteocalcin and the activity of alkaline phosphatase in C2C12 cells significantly increased, and the model of osteoblastic differentiation of C2Cl2 cells was established. The expression of SATB2 mRNA and SATB2 protein gradually increased with the progression of osteoblastic differentiation of C2Cl2 cells treated by 150 pfu/cell AdvBMP2, and the expression of SATB2 mRNA and SATB2 protein increased with the concentration increase of 0-225 pfu/cell Adv-BMP2. Conclusion BMP2 may influence osteoblastic differentiation of MSCs by regulating the expression of SATB2.

Key words: bone morphogenetic protein 2, osteoblast transcription factor, SATB2, gene expression, regulation