Oxidized low-density lipoprotein reduces macrophage phagocytosis of apoptotic cells by inhibiting expression of receptor MerTK

doi:10.3969/j.issn.1674-8115.2012.06.004

Abstract

Abstract:

Objective To investigate the effect and mechanism of macrophage phagocytosis of apoptotic cells (efferocytosis) treated by oxidized low-density lipoprotein (ox-LDL). Methods RAW264.7 murine macrophages were randomly divided into control group (treated by serum-free DMEM culture medium), 10 μg/mL ox-LDL group (treated by 10 μg/mL ox-LDL serum-free DMEM culture medium) and 20 μg/mL ox-LDL group (treated by 20 μg/mL ox-LDL serum-free DMEM culture medium). RAW264.7 murine macrophage apoptosis was induced by ultraviolet radiation, phagocytic index of RAW264.7 macrophages of apoptotic cells was determined by flow cytometry, and Western blotting and Real-Time PCR were employed to detect the expression of receptor MerTK protein and mRNA respectively. Results ①Twenty-four hours after treatment, the phagocytic indexes in 10 μg/mL ox-LDL group and 20 μg/mL ox-LDL group decreased by (4.7±2.8)% and (12.6±2.2)% respectively of that in control group, and the phagocytic index of 20 μg/mL ox-LDL group was significantly lower than that in control group and 10 μg/mL ox-LDL group （P<0.05). ②Twenty-four hours after treatment, the relative expression of MerTK protein in 10 μg/mL ox-LDL group and 20 μg/mL ox-LDL group decreased by (20.0±16.5)% and (47.0±15.4)% respectively of that in control group, and the relative expression of MerTK protein in 20 μg/mL ox-LDL group was significantly lower than that in control group （P<0.05). ③Twelve hours after treatment, the relative expression of MerTK mRNA in 10 μg/mL ox-LDL group and 20 μg/mL ox-LDL group decreased by (33.0±17.5)% and (60.0±10.0)% respectively of that in control group, and the relative expression of MerTK mRNA in 10 μg/mL ox-LDL group and 20 μg/mL ox-LDL group was significantly lower than that in control group （P<0.05). Conclusion ox-LDL can reduce the macrophage phagocytosis of apoptotic cells, and the mechanism may be related to inhibiting the expression of receptor MerTK, which may also be one of the causes for the instability and progression of ox-LDL-induced atherosclerotic plaque.

Key words: oxidized low-density lipoprotein, effrocytosis, macrophage, MerTK receptor

HUANG Xiao-jing, JIANG Li-sheng, XU Jin, et al. Oxidized low-density lipoprotein reduces macrophage phagocytosis of apoptotic cells by inhibiting expression of receptor MerTK[J]. , 2012, 32(6): 706-.

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Oxidized low-density lipoprotein reduces macrophage phagocytosis of apoptotic cells by inhibiting expression of receptor MerTK

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