上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2018, Vol. 38 ›› Issue (10): 1269-.doi: 10.3969/j.issn.1674-8115.2018.10.024

• 技术与方法 • 上一篇    下一篇

伐地那非预处理对精子冷冻效果的影响

卢克敏 1, 2，邹沙沙 2，陈向锋 2，刘强 1   

  1. 1. 上海交通大学基础医学院组织胚胎学与遗传发育学系，上海市生殖医学重点实验室，上海 200025；2. 上海交通大学医学院附属仁济医院生殖医学中心，上海市人类精子库，上海市辅助生殖与优生重点实验室，上海 200135
  • 出版日期:2018-10-28 发布日期:2018-11-18
  • 通讯作者: 刘强，电子信箱：qliu0122@shsmu.edu.cn。陈向锋，电子信箱：allanbacon@163.com。为共同通信作者。
  • 作者简介:卢克敏 (1988—)，男，初级技师，硕士生；电子信箱： lukemin999@163.com。
  • 基金资助:
    国家自然科学基金（ 81501243，81571488）；上海市卫生和计划生育委员会科研课题（ 201440009）；上海交通大学医学院附属仁济医院种子基金（RJZZ14-017）

Effect of vardenafil pretreatment on the activity of post-thaw sperm

LU Ke-min1, 2, ZOU Sha-sha2, CHEN Xiang-feng2, LIU Qiang1   

  1. 1. Shanghai Key Laboratory of Reproductive Medicine; Department of Histoembryology, Genetics and Developmental Biology, Shanghai Jiao Tong University College of Basic Medical Sciences, Shanghai 200025, China; 2. Center for Reproduction Medicine, Renji Hospital, Shanghai Jiao Tong University School of Medicine; Shanghai Human Sperm Bank; Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Shanghai 200135, China
  • Online:2018-10-28 Published:2018-11-18
  • Supported by:
    National Natural Science Foundation of China, 81501243, 81571488; Scientific Research Project of Shanghai Municipal Commission of Health and Family Planning, 201440009; Foundation of Renji Hospital, Shanghai Jiao Tong University School of Medicine, RJZZ14-017

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摘要： 目的 ·探索伐地那非预处理对精子冷冻复苏后活力的影响。方法 ·在上海市人类精子库随机收集 30例捐献者的精液标本，并将收集的每例精液平均分成 6份，1份用于冷冻前指标测定，其余 5份分别用 0、0.4、4.0、40.0、400.0 μg/mL的伐地那非孵育 5 min。按精液与保护剂 2:1的比例添加保护剂，采用一步熏蒸法进行冷冻，冷冻复苏后分别检测每份标本的前向运动精子、精子活力及正常精子形态等参数。结果 ·复苏后， 0、0.4、4.0、40.0、400.0 μg/mL伐地那非处理后的前向运动精子百分率分别为（ 41.47±9.80）%、（42.57±9.60）%、（47.77±8.55）%、（37.27±8.47）%、（26.37±6.99）%。与对照组（ 0 μg/mL伐地那非组）比较， 4.0 μg/mL伐地那非处理后的精子复苏后活力有显著改善，差异有统计学意义（ P0.034）。结论 ·适当浓度的伐地那非预处理精液能提高精子冷冻复苏后的活力；然而伐地那非浓度过大，可能对精子有毒害作用。

关键词: 伐地那非, 冷冻保存, 精子活力

Abstract:

Objective · To explore the effect of vardenafil pretreatment on the post-thaw sperm activity. Methods · Semen samples were randomly collected 30 donors in Shanghai Human Sperm Bank. Then, each semen sample was aliquoted into six parts. One was immediately assayed for sperm activity without any treatment as the original control. The other five parts were incubated with vardenafil at 0, 0.4, 4.0, 40.0, and 400.0 μg/mL respectively for five minutes. The protective agent was added according to the semen: protective agent ration (2:1), and the fumigation method was used for cryopreservation. After thawing, each specimen was examined for various parameters, including progressive motility, total motility and normal sperm morphology. Results · After thawing, the progressive sperm rates were (41.47±9.80)%, (42.57±9.60)%, (47.77±8.55)%, (37.27±8.47)%, and (26.37±6.99)% in the groups treated with 0, 0.4, 4.0, 40.0, and 400.0 μg/mL of vardenafil, respectively. Compared to the control group (0 μg/mL of vardenafil), 4.0 μg/mL of vardenafil could significantly improve the post-thaw sperm motility (P0.034). Conclusion · Vardenafil pretreatment can improve the activity of the post-thaw sperm; however, it may be toxic to sperm at the high concentration.

Key words: vardenafil, cryopreservation, sperm motility

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