上海交通大学学报（医学版）

上海交通大学学报(医学版) ›› 2020, Vol. 40 ›› Issue (09): 1157-1167.doi: 10.3969/j.issn.1674-8115.2020.09.001

• 论著·基础研究 • 上一篇    下一篇

核酸适配体sgc8在急性白血病诊断中的应用研究

徐康力1，马亚妮2，王筱金3，苗彦彦1，韩 达1#，谭蔚泓1#   

  1. 1.上海交通大学医学院附属仁济医院分子医学研究院，上海200127；2.上海交通大学医学院附属上海儿童医学中心，卫生部儿童血液肿瘤重点实验室，上海200127；3.上海交通大学医学院临床研究中心生物统计学教研室，上海 200025
  • 出版日期:2020-09-28 发布日期:2020-11-04
  • 通讯作者: 谭蔚泓，电子信箱：tan@hnu.edu.cn。韩 达，电子信箱：dahan@sjtu.edu.cn。#为共同通信作者。
  • 作者简介:徐康力（1988—），男，博士生；电子信箱：kanglix@gmail.com。
  • 基金资助:
    国家自然科学基金面上项目（21974087）；国家重大科研仪器研制项目（21827811）；上海市教育委员会高峰高原学科建设计划（20181709）；上海市青年科技启明星计划（20QA1405800）；上海交通大学医学院地方高水平大学建设项目。

Using aptamer of sgc8 for diagnosis of acute leukemia

XU Kang-li1, MA Ya-ni2, WANG Xiao-jin3, MIAO Yan-yan1, HAN Da1#, TAN Wei-hong1#   

  1. 1. Institute of Molecular Medicine, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China; 2. Key Laboratory of Ministry of Health for Children's Hematology and Oncology, Shanghai Children’s Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China; 3. Department of Biostatistics, Clinical Research Institute, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
  • Online:2020-09-28 Published:2020-11-04
  • Supported by:
    National Natural Science Foundation of China （21974087); National Key Scientific Instrument and Equipment Development Project (21827811); Shanghai Municipal Education Commission—Gaofeng Clinical Medicine Grant Support (20181709); Shanghai Rising-Star Program (20QA1405800); Local High-Level University Construction Project of Shanghai Jiao Tong University School of Medicine.

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摘要： 目的·研究核酸适配体sgc8 对不同骨髓细胞的识别能力，评价其对急性白血病（acute leukemia，AL)的诊断价值。方法·利用5-羧基荧光素标记的核酸适配体sgc8及其对照随机文库链（Lib）通过流式细胞术检测83例临床骨髓样本，分析各细胞群sgc8与Lib阳性百分比差值。采用Kruskal-Wallis检验进行统计学分析。结果·在AL样本中， sgc8在大部分急性T淋巴细胞白血病（T-cell acute lymphoblastic leukemia，T-ALL）和急性髓系细胞白血病（acute myeloid leukemia，AML）样本中对肿瘤细胞具有特异性识别能力；其中，sgc8对不同AML亚型肿瘤细胞的识别能力不同，而对T-ALL不同亚型肿瘤细胞的识别能力没有差异；但在急性B淋巴细胞白血病（B-cell acute lymphoblastic leukemia，B-ALL）样本中，sgc8未见明显识别能力。在正常骨髓样本中，sgc8可以识别髓系原始细胞，而不识别早期B细胞及其他成熟细胞群。结论·sgc8在AML、T-ALL和正常骨髓样本中具有特异性识别能力，且对不同AML亚型可能具有不同的诊断价值，但它对B-ALL样本的细胞无显著识别能力。

关键词: 核酸适配体, sgc8, 急性白血病, 流式细胞术, 诊断

Abstract:

Objective · To study the ability of aptamer sgc8 to recognize different bone marrow cells and evaluate its diagnostic value for acute leukemia (AL). Methods · 5-Carboxyfluorescein-labeled aptamer sgc8 and its control random library chain (Lib) were used to examine 83 clinical bone marrow samples by flow cytometry, and the difference between sgc8 and Lib of the positive percentages of each cell population was analyzed. Kruskal-Wallis test was used for statistical analysis. Results · In AL samples, sgc8 has a specific ability to recognize tumor cells in most of the acute T-cell acute lymphoblastic leukemia (T-ALL) and acute myeloid leukemia (AML) samples, and the ability of sgc8 to recognize tumor cells of various AML subtypes is different while there is no difference for the ability to recognize tumor cells of T-ALL subtypes. But sgc8 doesn’t show obvious recognition ability in acute B-cell lymphoblastic leukemia (B-ALL) samples. In normal bone marrow samples, sgc8 can specifically recognize the myeloid blast cellsbut not early B cells and other mature cell populations. Conclusion · The aptamer sgc8 has specific recognition ability in AML, T-ALL and normal bone marrow samples, and may have different diagnostic values for various AML subtypes, but it does not significantly recognize cells of B-ALL samples.

Key words: aptamer, sgc8, acute leukemia, flow cytometry, diagnosis

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