›› 2009, Vol. 29 ›› Issue (12): 1447-.

• 论著(基础研究) • 上一篇    下一篇

手术创伤动员血管内皮祖细胞入血与移植瘤生长的关系

王国江1, 戴 强1, 张燕捷1, 吴云林2   

  1. 上海交通大学 医学院 1. 第三人民医院消化科, 上海 201900;2. 瑞金医院消化科, 上海 200025
  • 出版日期:2009-12-25 发布日期:2009-12-25
  • 通讯作者: 吴云林, 电子信箱: wuyunlinruijin@163.com。
  • 作者简介:王国江(1979—), 男, 硕士生;电子信箱: moshiwang2000@163.com。
  • 基金资助:

    上海市科委“科技创新行动计划”基础研究重点项目(07jc14042)

Correlation of tumor growth and endothelial progenitor cells entering blood induced by surgical injury

WANG Guo-jiang1, DAI Qiang1, ZHANG Yan-jie1, WU Yun-lin2   

  1. 1. Department of Gastroenterology, The Third People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 201900;2. Department of Gastroenterology, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200025
  • Online:2009-12-25 Published:2009-12-25
  • Supported by:

    "Action Projects of Scientific and Technical Innovation" of Major Foundamental Research Program of Shanghai Science and Technology Committee, 07jc14042

摘要:

目的 研究手术创伤动员荷瘤裸鼠血管内皮祖细胞(EPC)入血与实体瘤生长的关系。方法 将42只荷瘤裸鼠随机分为非手术1 d组、单纯麻醉组、手术创伤组(术后24 h、48 h、72 h、30 d组)及非手术30 d组,每组6只。单纯麻醉组24 h后取血及获取移植瘤组织,手术创伤组分别在术后24 h、48 h、72 h、30 d取血及获取移植瘤组织。流式细胞仪检测各组外周血EPC百分比;ELISA检测血清血管内皮生长因子(VEGF)水平;免疫组织化学法检测移植瘤组织VEGF表达及微血管密度(MVD)。结果 术后24 h、48 h、72 h组EPC百分比与非手术1 d组比较,差异均有统计学意义(P<0.05);术后24 h组、48 h组、72 h组、单纯麻醉组VEGF水平与非手术1 d组比较,差异有统计学意义(P<0.05);各组MVD差异无统计学意义(P>0.05)。Pearson相关性分析显示,血清VEGF水平与外周血EPC百分比呈正相关(r=0.695 6,P<0.01),外周血EPC百分比与MVD无相关性(r=0.221 4,P>0.05),血清VEGF水平与MVD也无相关性(r=0.224 9,P>0.05)。结论 手术创伤对移植瘤生长无明显促进作用。

关键词: 移植瘤, 手术创伤, 血管内皮祖细胞, 微血管密度

Abstract:

Objective To explore the correlation of tumor growth and endothelial progenitor cells (EPC) entering blood induced by surgical injury in tumor bearing nude mice. Methods Forty-two tumor bearing nude mice were randomly divided into seven groups (n=6): non-surgical injury groups (1 d and 30 d), anesthetic group, surgical injury groups (24 h, 48 h, 72 h and 30 d after surgery). Blood samples and xenograft tumor tissues were taken from anesthetic group 24 h after anaesthesia and surgical injury groups 24 h, 48 h, 72 h and 30 d after surgery. EPC levels in peripheral blood were measured by flow cytometry, serum VEGF levels were determined by ELISA, microvessel density (MVD) and expression of VEGF were detected by immunohistochemistry. Results The levels of EPC in 24 h post-surgery group, 48 h post-surgery group and 72 h post-surgery group were significantly higher than that in non-surgical injury 1 d group (P<0.05). The levels of VEGF in 24 h post-surgery group, 48 h post-surgery group, 72 h post-surgery group and anesthetic group were significantly higher than that in non-surgical injury 1 d group (P<0.05). There was no significant difference in MVD among groups (P>0.05). Pearson correlation analysis revealed that serum VEGF levels were related to EPC levels in peripheral blood (r=0.695 6, P<0.01), while EPC levels in peripheral blood were not related to MVD (r=0.221 4, P>0.05), and serum VEGF levels had no correlation with MVD (r=0.224 9, P>0.05). Conclusion Surgical injury has no obvious influence on xenograft tumor growth.

Key words: xenograft, surgical injury, endothelial progenitor cell, microvessel density