上海交通大学学报（医学版）

›› 2011, Vol. 31 ›› Issue (6): 759-.doi: 10.3969/j.issn.1674-8115.2011.06.017

• 论著（基础研究） • 上一篇    下一篇

空间记忆维持期小鼠海马酪氨酸激酶B含量的变化

陆志俊1, 辛怡纯2, 陈 恳2, 于布为1   

  1. 上海交通大学 医学院附属瑞金医院 1.麻醉科, 2.卢湾分院麻醉科, 上海 200025
  • 出版日期:2011-06-28 发布日期:2011-06-27
  • 通讯作者: 于布为, 电子信箱: yubuwei@yahoo.com.cn。
  • 作者简介:陆志俊（1972—）, 男, 副主任医师, 博士；电子信箱: lusamacn@yahoo.com。
  • 基金资助:

    卢湾区医学基金（LWXK0902）

Content of tyrosine kinase B in hippocampus of mouse during persistence of spatial memory

LU Zhi-jun1, XIN Yi-chun2, CHEN Ken2, YU Bu-wei1   

  1. 1.Department of Anesthesiology, Ruijin Hospital, 2.Department of Anesthesiology, Ruijin Hospital Luwan Branch, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China
  • Online:2011-06-28 Published:2011-06-27
  • Supported by:

    Luwan District Medical Foundation, LWXK0902

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摘要：

目的 观察Morris水迷宫实验中小鼠记忆维持阶段海马酪氨酸激酶B（trkB）蛋白和mRNA的表达情况。方法 将28只C57BL/6品系小鼠分为实验组（n=14）和对照组（n=14），实验组参加水迷宫实验，包括4 d训练和1 d探索实验；对照组仅参加1 d探索实验。记录训练期小鼠的潜伏期、游泳距离和平均游泳速度；探索实验中记录小鼠穿越平台次数和平台象限滞留时间百分比。探索实验结束后采用ELISA法测定小鼠海马trkB蛋白含量，采用Real-Time PCR法测定trkB mRNA表达。结果 水迷宫训练期，实验组小鼠潜伏期和游泳距离随训练次数增加逐渐缩短(P<0.05)；探索实验中实验组小鼠穿越平台次数和平台象限滞留时间百分比明显高于对照组(P<0.05)；两组小鼠海马trkB蛋白和mRNA表达差异均无统计学意义(P>0.05)。结论 Morris水迷宫实验显示，小鼠形成对平台空间位置的长期记忆；但在记忆维持阶段，小鼠海马trkB合成没有增加。

关键词: Morris水迷宫, 空间记忆, 酪氨酸激酶B

Abstract:

Objective To investigate the expression of tyrosine kinase B (trkB) protein and mRNA in hippocampus of mouse during persistence of spatial memory in Morris water maze. Methods Twenty-eight C57BL/6 mice were divided into test group (n=14) and control group (n=14). MWM were performed on mice in test group, which included four trail days and one probe day, and mice in control group only experienced one probe day. During trail days, the latencies, swimming distances and swimming velocities were recorded. On probe day, the times across platform and time percent during platform quadrant were recorded. All mice were sacrificed after probe, and hippocampi were dissected for determination of contents of trkB protein and expression of trkB mRNA by ELISA and Real-Time RCR respectively. Results During trail days, the latencies and swimming distances of mice in test group decreased after repetitive training (P<0.05). On probe day, the times across platform and time percent during platform quadrant in test group were significantly higher than those in control group (P<0.05). There was no significant difference in the expression of trkB protein and mRNA in hippocampi between test group and control group (P>0.05). Conclusion Long-term spatial memory of mouse is established in Morris water maze, but the content of trkB is not upregulated during persistence of spatial memory.

Key words: Morris water maze, spatial memory, tyrosine kinase B