上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

电压门控钠通道Nav1.6在骨癌痛模型大鼠背根神经节中的表达

范之丹1，马柯2，季晓燕3，程志军3   

  1. 上海交通大学 医学院附属新华医院 1.麻醉科， 2.疼痛科, 上海 200092； 3.上海交通大学 医学院附属新华医院崇明分院麻醉科， 上海 202150
  • 出版日期:2015-03-28 发布日期:2015-03-26
  • 通讯作者: 马柯, 电子信箱: marke72@163.com。
  • 作者简介:范之丹（1980—）， 女， 主治医师， 硕士生; 电子信箱: fanzhidan56@126.com。
  • 基金资助:

    上海市卫生和计划生育委员会基金（201440273）；崇明县科委基金（CKY2012-05）

Expression of voltage-gated sodium channel Nav1.6 in dorsal root ganglions of rat model of bone cancer pain

FAN Zhi-dan1, MA Ke2, JI Xiao-yan3, CHENG Zhi-jun3   

  1. 1.Department of Anesthesiology, 2.Pain Centre, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China; 3.Department of Anesthesiology, Xinhua Hospital (Chongming), Shanghai Jiao Tong University School of Medicine, Shanghai 202150, China
  • Online:2015-03-28 Published:2015-03-26
  • Supported by:

    Foundation of Municipal Commission of Health and Family Planning of Shanghai, 201440273；Chongming Science and Technology Committee Foundation， CKY2012-05

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摘要：

目的 建立大鼠胫骨癌痛模型，并观察电压门控钠通道亚型Nav1.6在其背根神经节（DRG）中的表达。方法 雌性Wistar大鼠随机分为3组：癌痛组（n=15）大鼠左胫骨骨髓腔内注射Walker256乳腺癌细胞，假手术组（n=10）注射生理盐水，以正常大鼠作为空白对照组（n=10）。于造模后第4、8、12、16、20日，观察大鼠体质量变化，评估机械性痛觉过敏以及热痛觉过敏。造模后20 d，取接种侧胫骨做病理切片，观察肿瘤生长情况，real-time PCR检测大鼠L5～L6 DRG中Nav1.6 mRNA的表达。结果 癌痛组大鼠出现进行性加重的疼痛行为学改变，癌痛组大鼠DRG中Nav1.6 mRNA的表达量明显高于假手术组和空白对照组（P<0.05）。结论 Walker256乳腺癌细胞制备的大鼠胫骨癌痛模型是骨转移瘤相关疼痛较可靠的模型。Nav1.6 mRNA在骨癌痛模型大鼠DRG中的表达上调，提示该通道可能参与骨癌痛的发生过程。

关键词: 骨癌痛, Nav1.6, 钠通道, 背根神经节

Abstract:

Objective To establish a rat model of bone cancer pain and observe the expression of voltage-gated sodium channel subtype Nav1.6 in dorsal root ganglions (DRG). Methods Female Wistar rats were randomly divided into 3 groups, i.e. the Walker256 group (n=15), sham group (n=10), and normal control group (n=10). The Walker256 group underwent intra-tibial injection of syngenetic Walker256 mammary gland carcinoma cells. The sham group received intra-tibial injection of normal saline. The normal Wistar rats served as normal controls. Changes of body weight were observed and pain thresholds of mechanical hyperalgesia and thermal hyperalgesia were assessed 4, 8, 12, 16, and 20 d after the rat pain model was established. Pathological sections of tibia that underwent the injection were prepared 20 d after the rat pain model was established. The development of the bone tumor was observed and the mRNA expression of Nav1.6 in L5-L6 DRG was detected by real-time PCR. Results The Walker256 group showed gradual development of both mechanical and thermal hyperalgesia. The mRNA expression of Nav1.6 in DRG of the Walker256 group was significantly higher than those of the sham group and normal control group (P<0.05). Conclusion The rat model of bone cancer pain established by intra-tibial injection of syngenetic Walker256 mammary gland carcinoma cells is a reliable pain model of bone metastases. The mRNA expression of Nav1.6 in DRG increases, which suggests that Nav1.6 may involve in the cancer-induced bone pain.

Key words: bone cancer pain, Nav1.6, sodium channel, dorsal root ganglion