上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

孕期边缘型维生素A缺乏对新生鼠RARα、Src和NMDAR1表达的影响

陈丽君1,2，任兰1，刘祖银1，李廷玉1,3，陈洁1，张萱1,3   

  1. 1.重庆医科大学附属儿童医院儿童发育疾病研究省部共建教育部重点实验室， 重庆 400014； 2.重庆医科大学公共卫生与管理学院， 重庆 400016； 3.重庆医科大学附属儿童医院儿童保健科， 重庆 400014
  • 出版日期:2015-04-28 发布日期:2015-04-29
  • 通讯作者: 张萱, 电子信箱: gracexuan330@163.com。
  • 作者简介:陈丽君（1989—）， 女， 硕士生; 电子信箱: chenlijuncq@163.com。
  • 基金资助:

    国家自然科学基金青年基金（81200483）；高等学校博士学科点专项科研基金(新教师类，20125503120006）

Effects of marginal vitamin A deficiency in pregnancy on expressions of retinoic acid receptor alpha, Src, and NMDAR1 of newborn rats

CHEN Li-jun1,2, REN Lan1, LIU Zu-yin1, LI Ting-yu1,3, CHEN Jie1, ZHANG Xuan1,3   

  1. 1.Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University， Chongqing 400014， China; 2.Public Health and Management School, Chongqing Medical University,Chongqing 400016， China; 3.Department of Primary Child Care, Children's Hospital, Chongqing Medical University, Chongqing 400014， China
  • Online:2015-04-28 Published:2015-04-29
  • Supported by:
    Young Scientists Project of National Natural Science Foundation of China, 81200483; Research Fund for the Doctoral Program of Higher Education of China， Type of New Teachers, 20125503120006

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摘要：

目的 探讨孕期边缘型维生素A缺乏（MVAD）对新生鼠海马中视黄酸核受体α(RARα)、肉瘤基因Src和N-甲基-D-天门冬氨酸受体1(NMDAR1)表达的影响。方法 构建孕期维生素A正常（VAN）和MVAD大鼠模型（VAN组和MVAD组）；分离培养新生鼠原代海马神经元，免疫荧光染色鉴定；采用real-time PCR和Western blotting检测新生鼠海马组织及体外培养的原代海马神经元中RARα、Src和NMDAR1的mRNA和蛋白表达；利用钙影像仪检测原代海马神经元钙兴奋性。结果 免疫荧光染色显示，分离培养的新生鼠原代海马神经元95%表达神经元标志物神经元特异性烯醇化酶（NSE）；MVAD组新生鼠海马组织和原代海马神经元中RARα、Src、NMDAR1的mRNA和蛋白表达水平明显低于VAN组（P<0.05）；MVAD组新生鼠海马神经元的钙兴奋性明显低于VAN组（P<0.05）。结论 孕期MVAD会降低海马RARα、Src和NMDAR1的表达，可能与孕期MVAD影响幼鼠的学习记忆功能有关。

关键词: 孕期, 边缘型维生素A缺乏, 视黄酸核受体α, 肉瘤基因, N-甲基-D-天门冬氨酸受体1

Abstract:

Objective To explore the marginal vitamin A deficiency (MVAD) in pregnancy on the expressions of retinoic acid receptor alpha (RARα), sarcoma gene Src, and N-methyl-D-aspartate receptor subunit 1 (NMDAR1) in the hippocampus of newborn rats. Methods The pregnant rat models of vitamin A normal (VAN group) and marginal vitamin A deficiency (MVAD group) were established. The hippocampus tissue of newborn rats was isolated. The primary hippocampus neurons were cultured in vitro and detected by the immunofluorescence staining. The mRNA and protein expressions of RARα, Src, and NMDAR1 in hippocampus tissue and primary hippocampus neurons of newborn rats were detected by the real-time PCR and Western blotting. The calcium excitability of primary hippocampus neurons was detected by the calcium imaging instrument. Results The results of immunofluorescence staining showed that 95% of primary hippocampus neurons of newborn rats that were isolated and cultured in vitro expressed the neuron maker of neuron-specific enolase (NSE). The mRNA and protein expressions of RARα, Src, and NMDAR1 in hippocampus tissue and primary hippocampus neurons of newborn rats of the MVAD group were significantly lower than those of the VAN group (P<0.05). The calcium excitability of hippocampus neurons of newborn rats of the MVAD group was significantly lower than that of the VAN group (P<0.05). Conclusion MVAD in pregnancy can decrease expressions of RARα, Src, and NMDAR1 in the hippocampus. This may be relevant to the impaired learning and memory functions of newborn rats caused by MVAD in pregnancy.

Key words: pregnancy, marginal vitamin A deficiency, retinoic acid receptor alpha, sarcoma gene, N-methyl-D-aspartate receptor subunit 1