上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2017, Vol. 37 ›› Issue (9): 1196-.doi: 10.3969/j.issn.1674-8115.2017.09.002

• 论著（基础研究） • 上一篇    下一篇

PRV 逆行示踪技术验证神经根移位重建大鼠排便功能的有效性

朱晓中，朱弘一，鲍丙波，李星玮，高 涛，郑宪友   

  1. 上海交通大学附属第六人民医院骨科，上海 200233
  • 出版日期:2017-09-28 发布日期:2017-10-10
  • 通讯作者: 郑宪友，电子信箱：zhengxianyou@126.com
  • 作者简介:朱晓中（1989—），男，住院医师，硕士；电子信箱：jukasjtu@126.com
  • 基金资助:
     国家自然科学基金（81371965，81672144）；上海市浦江人才计划（16PJD035）；上海市教育委员会高峰高原学科建设计划（20161429）

Effectiveness of defecation reconstruction following nerve transfer in rats by PRV retrograde tracing

ZHU Xiao-zhong, ZHU Hong-yi, BAO Bing-bo, LI Xing-wei, GAO Tao, ZHENG Xian-you   

  1. Department of Orthopaedics, Shanghai Sixth People’s Hospital, Shanghai Jiao Tong University, Shanghai 200233, China
  • Online:2017-09-28 Published:2017-10-10
  • Supported by:
    National Natural Science Foundation of China, 81371965, 81672144；Shanghai Pujiang Program, 16PJD035; Shanghai Municipal Education Commission—Gaofeng Clinical Medicine Grant Support, 20161429

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摘要：  目的 · 通过伪狂犬病毒（PRV）逆行示踪技术研究神经根移位术是否能够重建大鼠脊髓损伤后的排便功能。方法 · 取大鼠 20 只，于 S1、L6 神经根之间横断脊髓，10 只作为对照（A 组）， 10 只行神经根移位手术（B 组）。 6 个月后，每只大鼠注射 6 μL PRV， 注射 3 d 后用多聚甲醛灌注，取出脊髓，冰冻包埋后切片观察 PRV 阳性标记情况。结果 · A 组于损伤平面以上脊髓切片中未发现 PRV 阳性标记，而 B 组于损伤平面以上脊髓切片中发现被 PRV 感染的神经元细胞。结论 · 神经根移位在大鼠脊髓损伤后排便功能的重建 中具有明确作用，PRV 逆行示踪技术可验证新的反射通路的存在。

关键词: 伪狂犬病毒, 神经示踪, 脊髓损伤, 排便反射

Abstract:

Objective · To investigate the effectiveness of nerve transfer in repairing defecation function after spinal cord injury by the pseudorabies virus (PRV) retrograde tracing.  Methods · The spinal cords were transected between L6 and S1 nerve root in 20 rats. The nerve transferring surgery was then conducted in 10 rats (Group B) and the remaining rats were control (Group A). After six months, all rats were injected with 6 μL PRV, sacrificed after 3 d and perfused with paraformaldehyde. Spinal cords were then harvested and frozen sections were prepared for observation.  Results · There was no detectable infection of PRV proximal to the injury level in Group A, while infected neurons proximal to the injury level were widely observed in Group B.  Conclusion · Nerve transfer has potent effect on defecation reconstruction after spinal cord injury in rats. PRV retrograde tracing can prove the existence of new neuron pathway.

Key words: pseudorabies virus, nerve tracing, spinal cord injury, defecation reflex