上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2018, Vol. 38 ›› Issue (3): 259-.doi: 10.3969/j.issn.1674-8115.2018.03.004

• 论著（基础研究） • 上一篇    下一篇

Nd: YAG 激光对具核梭杆菌的杀灭作用

吴一凡，宋忠臣，束 蓉   

  1. 上海交通大学 医学院附属第九人民医院牙周科，上海市口腔医学重点实验室，上海 200011
  • 出版日期:2018-03-28 发布日期:2018-05-03
  • 通讯作者: 束 蓉，电子信箱：shurong1977@163.com
  • 作者简介:吴一凡（1991—），男，住院医师，硕士生；电子信箱：wuyifan10shanghai@163.com
  • 基金资助:
     国家自然科学基金（81570977）

Antibacterial effect of Nd: YAG laser on Fusobacterium nucleatum

WU Yi-fan, SONG Zhong-chen, SHU Rong   

  1. Shanghai Key Laboratory of Stomatology, Department of Periodontology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China
  • Online:2018-03-28 Published:2018-05-03
  • Supported by:
    National Nature Science Foundation of China, 81570977

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摘要： 目的 · 观察 Nd: YAG 激光对具核梭杆菌（Fusobacterium nucleatum，F. nucleatum）的杀灭效果。方法 · 使用结晶紫染色，观
察 Nd: YAG 激光对浮游状态 F. nucleatum 成膜能力的影响；运用激光共聚焦显微镜扫描分析，观察激光对单菌种生物膜中 F. nucleatum
活力的影响；使用扫描电子显微镜技术，观察激光对单菌种生物膜中 F. nucleatum 结构的破坏。结果 · 激光照射 15 s 对浮游状态下
F. nucleatum 成膜能力有抑制作用，激光照射 35 s 对生物膜状态下 F. nucleatum 活力产生抑制。激光能破坏生物膜及生物膜中细菌的结
构，且随光照时间延长，破坏程度增加。结论 · Nd: YAG 激光能够抑制浮游状态 F. nucleatum 的成膜能力，对单一菌种生物膜中细菌
活力和细菌结构产生破坏，从而达到杀菌效果。

关键词: &ensp, 牙周致病菌；生物膜；Nd: YAG 激光；体外实验

Abstract:

Objective · To investigate the antibacterial effect of Nd: YAG laser on Fusobacterium nucleatum (F. nucleatum) in vitro. Methods · The
laser effect on the biofilm formation ability of planktonic F. nucleatum were observed by crystal violet test; confocal laser scanning microscopy (CLSM)
was used to detect the effect on the bacteria viability of mature biofilm; scanning electron microscopy (SEM) was performed to investigate the effect on
the morphology of mature bioflm. Results · After 15 s radiation the biofilm formation ability of F. nucleatum went down. The viability of F. nucleatum
in mature biofilm went down under 35 s iradiation. The deformation of mature biofilm and bacterium became distincted with the iradiation time raised.
Conclusion · Nd: YAG laser shows the inhibition of biofilm formation ability on planktonic F. nucleatum. The vitality of F. nucleatum in mature biofilm is
depressed after laser radiation. Laser can destroy the construction of the mature biofilm and bacteria.

Key words: periodontal pathogens, biofilm, Nd: YAG laser, in vitro experiment