上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2019, Vol. 39 ›› Issue (9): 940-.doi: 10.3969/j.issn.1674-8115.2019.09.002

• 论著·基础研究 • 上一篇    下一篇

孕期精神压力与铅复合暴露对子代大鼠恐惧性记忆的影响

黄丽桦 1, 2，徐健 1, 2，张怡静 1, 2，刘军霞 1   

  1. 1. 上海交通大学医学院附属新华医院教育部及上海市环境与儿童健康重点实验室，上海 200092；2.上海交通大学医学院附属国际和平妇幼保健院儿童保健科，上海 200030
  • 出版日期:2019-09-28 发布日期:2019-11-02
  • 通讯作者: 徐健，电子信箱：sonia0616@sjtu.edu.cn。
  • 作者简介:黄丽桦（1992—），女，硕士生；电子信箱： huanglihua223@qq.com。
  • 基金资助:
    国家自然科学基金面上项目（81673189，30901205）；上海市教育委员会高峰高原学科建设计划（20172016）；上海市卫生和计划生育委员会项目（201640363）

Impact of maternal gestation combined exposure to lead and mental stress on offsprings fear-conditioning memory

HUANG Li-hua1, 2, XU Jian1, 2, ZHANG Yi-jing1, 2, LIU Jun-xia1   

  1. 1. MOE & Shanghai Key Lab of Childrens Environmental Health, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China; 2.The International Peace Maternity & Child Health Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200030, China
  • Online:2019-09-28 Published:2019-11-02
  • Supported by:
    National Natural Science Foundation of China, 81673189, 30901205; Shanghai Municipal Education Commission—Gaofeng Clinical Medicine Support, 20172016; Project of Shanghai Municipal Commission of Health and Family Planning, 201640363）。

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摘要： 目的 ·在孕期铅暴露的基础上，探讨孕期精神压力是否对铅在子代的血液、骨骼中的蓄积有增强作用；探讨孕期压力、铅和铅 –压力复合暴露对子代恐惧性记忆的影响。方法 ·将孕鼠随机分为对照组、压力暴露组、铅暴露组、铅 –压力暴露组，分娩后每组保留仔鼠 12只，雌雄各半。子代大鼠 3周龄时通过条件恐惧实验评估仔鼠对条件性恐惧记忆的消退过程， 4周龄时处死子代大鼠，提取静脉血、胫骨，以原子吸收光谱法检测血铅水平，以电感耦合等离子质谱仪检测骨铅水平。运用方差分析比较各组间血铅、骨铅及恐惧记忆水平，运用 Logistic回归模型分析血铅、骨铅与恐惧记忆的关系。结果 ·铅暴露组（ P血铅 0.013，P骨铅 0.000）、铅 –压力复合暴露组（ P0.000，P0.000）血铅和骨铅水平均高于对照组。压力暴露组血铅水平略高于对照组（ P0.056），但差异血铅骨铅 血铅 无统计学意义；压力暴露组骨铅水平显著高于对照组（ P0.004）。铅 –压力复合暴露的血铅、骨铅水平较铅暴露组有所上升，但骨铅 差异无统计学意义（ P血铅 0.682，P骨铅 0.124）。与低血铅组、低骨铅组相比，更高的血铅 /骨铅组的幼鼠在条件恐惧测试中第二时间段（P0.008/P0.016）和第三时间段（ P0.019/P0.005）高恐惧反应的可能性显著增大。铅 –压力复合暴露组在条件恐惧测试中第一时间段（[ 83.73±25.47）%]、第二时间段（[ 92.97±15.75）%]的僵直时间比率分别显著高于对照组第一时间段（[ 65.35±28.80）%， P10.048]和第二时间段（[ 68.78±27.22）%，P20.021]。结论 ·孕期铅 –压力复合暴露较铅暴露有进一步升高子代血铅、骨铅的趋势。母孕期铅暴露抑制子代大鼠恐惧性记忆消退过程，且这一效应可能被复合的压力暴露所加强。

关键词: 孕期压力, 铅暴露, 铅 &, ndash, 压力复合暴露, 条件恐惧

Abstract:

Objective · To explore whether prenatal stress can enhance the accumulation of blood/bone lead in the offspring on the basis of prenatal lead exposure, and to compare the effects of prenatal single exposures to stress/lead and prenatal combined exposure to lead and stress on fear-conditioning memory in the offspring. Methods · Pregnant rats were randomly divided into control group, stress exposure group, lead exposure group and combined lead-stress exposure group. After delivery, each group contained twelve pups (male:female1:1). The extinction process of fear-conditioning memory was evaluatedthe fear-conditioning test in the offspring at 3 weeks old. The offspring were then sacrificed at 4 weeks old. Blood and tibia samples were collected, blood lead was measuredusing the atomic absorption spectrometer, and tibia lead was measuredusing the inductively coupled plasma–mass spectrometry. The levels of blood lead, bone lead and fear memory were comparedanalysis of variance, and the relationship between blood lead, bone lead and fear memory were analyzedLogistic regression model. Results · The levels of blood and bone lead in the lead exposure group (P blood lead0.013, P bone lead0.000) and combined exposure group (P blood lead0.000, P bone lead0.000) were significantly higher than those in the control group; the level of blood lead in the stress group was higher but not significantly different that in the control group (P blood lead0.056) and the level of bone lead in the stress group was significantly higher than that in the control group (P bone lead0.004); the levels of blood and bone lead in combined exposure group were higher than those in the lead exposure group, but the differences didn’t reach statistical significance (P blood lead0.682, P bone lead0.124). Compared with young rats in the lowest blood lead/bone lead groups, young rats in the groups of higher blood/bone lead levels had higher odds ratios of high fear reaction during the second (P0.008/ P0.016) and the third (P0.019/P0.005) time periods. The ratios of freezing time in the first [(83.73±25.47)%] and the second [(92.97±15.75)%] periods of the fear-conditioning test in the combined exposure group were significantly higher than those in the control group [the first period, (65.35±28.80)%, P10.048; the second period, (68.78±27.22)%, P20.021]. Conclusion · Compared with the single exposure to lead during pregnancy, maternal gestation combined exposure to lead and stress may induce more increases in the blood and bone lead levels in the offspring. Lead exposure during pregnancy may inhibit the process of the extinction of fear memory in the offspring, and this effect may be aggravatedprenatal concurrent exposure to stress.

Key words: prenatal stress, lead exposure, combined lead-stress exposure, fear-conditioning memory

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