唾液菌群微生物与宿主血脂水平的关联分析

doi:10.3969/j.issn.1674-8115.2021.04.005

上海交通大学学报(医学版) ›› 2021, Vol. 41 ›› Issue (4): 442-447.doi: 10.3969/j.issn.1674-8115.2021.04.005

唾液菌群微生物与宿主血脂水平的关联分析

赵芬¹(), 晋巧巧¹, 苑克勇¹, 侯秀秀¹, 黄正蔚¹(), 马瑞¹^,²()

^1.上海交通大学医学院附属第九人民医院·口腔医学院牙体牙髓科，国家口腔疾病临床研究中心，上海市口腔医学重点实验室，上海市口腔医学研究所，上海 200011
^2.上海交通大学医学院附属第九人民医院·口腔医学院浦东分院，上海 200125

收稿日期:2020-07-05 出版日期:2021-04-28 发布日期:2021-05-14
通讯作者: 黄正蔚,马瑞 E-mail:925629949@qq.com;huangzhengwei@shsmu.edu.cn;marui1723@sina.com
作者简介:赵芬（1995—），女，硕士生；电子信箱：925629949@qq.com
基金资助:
国家自然科学基金(81570964)

Correlation analysis of salivary microbiome and host blood lipid levels

Fen ZHAO¹(), Qiao-qiao JIN¹, Ke-yong YUAN¹, Xiu-xiu HOU¹, Zheng-wei HUANG¹(), Rui MA¹^,²()

^1.Department of Endodontics, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai 200011, China
^2.Pudong Branch of Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200125, China

Received:2020-07-05 Online:2021-04-28 Published:2021-05-14
Contact: Zheng-wei HUANG,Rui MA E-mail:925629949@qq.com;huangzhengwei@shsmu.edu.cn;marui1723@sina.com
Supported by:
National Natural Science Foundation of China(81570964)

摘要/Abstract

摘要：

目的·分析唾液菌群微生物与宿主血脂水平的相关性。方法·采集114名45~60岁志愿者的唾液菌群样本，提取总DNA，随后应用多聚酶链式反应进行16S rDNA V3?V4区扩增。扩增所得产物经Illumina MiSeq PE300平台测序后，将所得序列进行操作分类单元（operational taxonomic units，OTUs）聚类、物种注释并将唾液菌群中丰度前50的属与宿主血脂水平作Spearman相关分析，P<0.05记为差异有统计学意义。结果·114名志愿者的唾液菌群样本测序所得平均序列数为（41 084±4 740）条，聚类所得OTUs数为1 153，共注释到23个门、43个纲、89个目、147个科和317个属。门水平和属水平的物种聚类热图示114个唾液菌群样本具有相似的物种组成丰度模式。Spearman相关分析结果显示，在唾液菌群微生物丰度前50的属中，Neisseria spp.的丰度与宿主血清总胆固醇（total cholesterol，TCH）、三酰甘油（triacylglycerol，TAG）和低密度脂蛋白胆固醇（low density lipoprotein cholesterol，LDL-Ch）的水平成负相关；Gemella spp.的丰度与宿主血清TCH、LDL-Ch水平呈正相关；与宿主血清高密度脂蛋白胆固醇水平成负相关的属有Comamonas spp.、Filifactor spp.和Parvimonas spp.。结论·人群中唾液菌群微生物的组成结构具有相对稳定性且与宿主血脂水平相关，可进一步挖掘唾液菌群微生物作为个体脂代谢异常的预警指标。

关键词: 唾液菌群, 血脂水平, 16S rDNA测序, Spearman相关分析

Abstract:

Objective·To analyze the correlation between salivary microbiome and host blood lipid levels.

Methods·Samples of saliva from 114 volunteers aged 45?60 years were collected to extract total DNA, and then 16S rDNA V3?V4 region was amplified with polymerase chain reaction. After the amplified products were sequenced via Illumina MiSeq PE300 platform,the obtained sequences were subjected to operational taxonomic units (OTUs) clustering and species annotation.The Spearman correlation analysis was also carried out to find the correlations between the top 50 abundant genera in the salivary microbiome and host blood lipid levels, P<0.05 was considered as statistically significant difference.

Results·The saliva samples of 114 volunteers were sequenced with an average sequence number of 41 084±4 740, and the number of OTUs obtained by clustering was 1 153, annotated to 23 phyla, 43 classes, 89 orders, 147 families and 317 genera. Species clustering heat map of 114 samples suggested that salivary microbiome has similar abundance patterns of species composition in the population.Spearman correlation analysis showed that among triacylglycerol (TAG) the top 50 abundant genera in the salivary microbiome, Neisseria spp. were negatively correlated with the host serum total cholesterol (TCH), and low density lipoprotein cholesterol level (LDL-Ch); Gemella spp. are positively correlated with Host serum TCH and LDL-Ch; high-density lipoprotein cholesterol presents negative correlation with Comamonas spp., Filifactor spp. and Parvimonas spp.

Conclusions·The composition structure of salivary microbiota in the population is quite stable and is related to the levels of host blood lipids. It is promising to further explore salivary microbiota as early warning indicators of individual lipid metabolism abnormalities.

Key words: salivary microbiome, blood lipid levels, 16S rDNA sequencing, Spearman correlation analysis

中图分类号:

R781.6

赵芬, 晋巧巧, 苑克勇, 侯秀秀, 黄正蔚, 马瑞. 唾液菌群微生物与宿主血脂水平的关联分析[J]. 上海交通大学学报(医学版), 2021, 41(4): 442-447.

Fen ZHAO, Qiao-qiao JIN, Ke-yong YUAN, Xiu-xiu HOU, Zheng-wei HUANG, Rui MA. Correlation analysis of salivary microbiome and host blood lipid levels[J]. JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE), 2021, 41(4): 442-447.

图/表 4

表1 研究对象的一般情况及血脂水平

Table 1 Demographic and clinical characteristics of the study participants

Parameter	Study participants (n=114)
Age/year	53.20±4.70
Gender/n(%)
Male	50 （43.86）
Female	64 （56.14）
TCH/(mmol·L^-1)	5.59±0.91
TAG/(mmol·L^-1)	1.80±1.07
LDL-Ch/(mmol·L^-1)	3.33±0.62
HDL-Ch/(mmol·L^-1)	1.29±0.29

图1 门水平的物种丰度聚类热图

Fig 1 Community heat map at phylum level

图2 属水平的物种丰度聚类热图

Fig 2 Community heat map at genus level

图3 唾液菌群微生物中丰度前50的属与宿主血脂水平的Spearman相关分析热图。

Fig 3 Heatmap of Spearman correlation analysis between the top 50 abundant genera of salivary microbiome and the blood lipid levels of hosts

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唾液菌群微生物与宿主血脂水平的关联分析

Correlation analysis of salivary microbiome and host blood lipid levels

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