上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

交联人脱细胞羊膜修复兔阴道黏膜缺损的研究

许培荣1, 吴氢凯2, 程 慧2，戴 淼2，侯 旻2，滕银成2   

  1. 1.苏州大学医学院研究生部， 苏州 215123； 2.上海交通大学附属第六人民医院妇产科， 上海 200233
  • 出版日期:2013-08-28 发布日期:2013-09-16
  • 通讯作者: 吴氢凯, 电子信箱: angelh2@163.com。
  • 作者简介:许培荣（1987—），女，硕士生; 电子信箱: 545172305@qq.com
  • 基金资助:

    上海交通大学医工（理）交叉研究基金（YG2010MS47）

Study on repair of vaginal mucosa defect by linked human acellular amniotic membrane in rabbits

XU Pei-rong1, WU Qing-kai2, CHENG Hui2, DAI Miao2, HOU Min2, TENG Yin-cheng2   

  1. 1.Department of Graduate Studies, Soochow University, Suzhou 215123, China; 2.Department of Obstetrics and Gynecology, the Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 200233, China
  • Online:2013-08-28 Published:2013-09-16
  • Supported by:

    Shanghai Jiaotong University Foundation, YG2010MS47

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摘要：

目的 探讨交联人脱细胞羊膜（HAM）修复兔阴道黏膜缺损的可行性。方法 采用兔脂肪干细胞（ADSCs）对交联HAM进行体外细胞毒性实验。将16只成年雌性新西兰大白兔随机分为实验组和对照组(n=8)，制作兔阴道后壁黏膜缺损模型，实验组用交联HAM覆盖缺损处，对照组不作任何处理。分别于术后3 d和1、2、4周时取全层阴道后壁组织行大体观察及HE染色评价阴道黏膜缺损的修复情况。结果 交联HAM浸提液吸光度值与阴性对照差异无统计学意义（P>0.05），细胞毒性0-Ⅰ级，交联HAM符合医用生物材料的细胞毒性要求。两组实验动物均未发生伤口感染。HE染色示术后3 d、1周，两组均可见较多炎性细胞浸润，但实验组可见较多阴道上皮细胞及平滑肌生成；术后2周，实验组阴道壁缺损处已覆盖新生上皮，而对照组阴道壁缺损处上皮覆盖不完整；术后4周，实验组阴道壁缺损处与周围正常组织无明显区别，而对照组新生阴道上皮细胞排列紊乱。结论 交联HAM可促进兔阴道黏膜缺损修复。

关键词: 人脱细胞羊膜, 创面修复, 阴道壁缺损, 细胞毒性试验

Abstract:

Objective To investigate the feasibility of repair of vaginal mucosa defect by linked human acellular amniotic membrane (HAM) in rabbits. Methods Adipose-derived stem cells (ADSCs) were used as the experimental cells to evaluate the cytotoxicity of linked HAM in vitro. Sixteen adult female New Zealand white rabbits were randomly divided into experiment group and control group (n=8), and vaginal mucosa defect models were prepared in two groups. The vaginal mucosa defect in experiment group was managed with linked HAM, while no treatment was conducted in control group. Full-thickness tissues of posterior vaginal wall were obtained 3 d, 1 week, 2 weeks and 4 weeks after surgery, and gross observation and HE staining were performed to evaluate the repair of vaginal mucosa defect. Results There was no significant difference in the absorbance value between linked HAM solution and negative controls (P>0.05), and the cytotoxicity graded 0 to I, which indicated that linked HAM met the standard for medical biomaterials in cytotoxicity. No wound infection was found in two groups of rabbits. Three days and 1 week after surgery, there was more inflammatory cell infiltration in two groups, and more proliferation of vaginal epithelial cells and smooth muscle development were found in experiment group. Two weeks after surgery, the vaginal mucosa defect was covered by a layer of vaginal epithelial tissues in experiment group, while only part of the vaginal epithelium covered the defect in control group. Four weeks after surgery, there was no significant difference between vaginal mucosa defect and surrounding normal tissues in experimental group, while the newly generated vaginal epithelial cells grew disorderly in control group. Conclusion Linked HAM may be the favorable material in repair of vaginal mucosa defect in rabbits.

Key words: human acellular amniotic membrane, wound repair, defect of vaginal mucosa, cytotoxicity test