上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

去铁胺通过促进毛细血管的增生改善放疗后唾液腺功能

张珺晔1,2，徐珉华2，郑元俐1   

  1. 1.上海交通大学 医学院附属第九人民医院口腔医学院口腔特需科 上海市口腔研究所 上海市口腔医学重点实验室， 上海 200011； 2.上海交通大学附属第一人民医院分院口腔科， 上海 200081
  • 出版日期:2013-12-28 发布日期:2014-01-02
  • 通讯作者: 郑元俐, 电子信箱: zhengyuanli@yahoo.com。
  • 作者简介:张珺晔（1980—）， 女， 主治医师， 硕士生; 电子信箱: biscuitmomo@126.com。

Preventive effects of deferoxamine on radiation damage to salivary glands by angiogenesis

ZHANG Jun-ye1,2, XU Min-hua2, ZHENG Yuan-li1   

  1. 1.Department of Oral Medicine， the Ninth People's Hospital， College of Stomatology， Shanghai Jiaotong University School of Medicine, Shanghai Research Institute of Stomatology, Shanghai Key Laboratory of Stomatology， Shanghai 200011， China； 2.Department of Stomatology, Branch of the First People's Hospital, Shanghai 200081, China
  • Online:2013-12-28 Published:2014-01-02

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摘要：

目的 探讨去铁胺（DFO）对放射治疗（放疗）后唾液腺功能的改善作用及可能机制。方法 C57BL/6小鼠随机分为正常对照组、单纯放疗组以及D+IR组（注射DFO 3 d后放疗）、D+IR+D组（注射DFO 3 d后放疗，再注射DFO 3 d）和IR+D组（放疗后注射DFO 3 d）及其相应对照组（不注射DFO而给予注射型蒸馏水）。放疗后第30、60、90天测定小鼠唾液流量；放疗后第90天处死小鼠，取下颌下腺，称取质量，行组织形态学和免疫组织化学检查，Western blotting测定缺氧诱导因子1α(HIF-1α)蛋白表达，Real-Time PCR检测血管内皮生长因子(VEGF)mRNA表达。结果 与相应对照组比较，D+IR、D+IR+D和IR+D组小鼠的唾液流量均显著增加（P<0.05），以D+IR+D最为显著。D+IR和D+IR+D组小鼠下颌下腺组织中可见少量唾液干细胞。D+IR、D+IR+D和IR+D下颌下腺HIF-1α蛋白和VEGF mRNA的表达均显著高于相应对照组（P<0.05）。结论 DFO对放疗后的唾液腺功能具有改善作用，其机制可能与促进毛细血管形成有关。

关键词: 唾液腺, 放射治疗, 去铁胺

Abstract:

Objective To investigate the efficacy of deferoxamine (DFO) for the regeneration of radiation damaged salivary glands. Methods C57BL/6 mice were divided into normal control group, irradiation group, D+IR group (DFO 3 d+irradiation), D+IR+D (DFO 3 d+irradiation+DFO 3 d), IR+D (irradiation+DFO 3d), and relative control groups (distilled water injection). Saliva flow rates were measured on day 30, 60, and 90 after irradiation. On day 90 after irradiation, the mice were sacrificed. Submandibular glands were resected and weighed, then, followed by histological and immunohistochemical analysis. Real-Time PCR and Western blotting were performed to measure expressions of HIF-1α protein and VEGF mRNA. Results Compared to relative control groups, saliva flow rates increased remarkably in D+IR, D+IR+D, and IR+D groups (P<0.05), where the highest was in D+IR+D group. In D+IR and D+IR+D groups, a small amount of saliva stem cells were observed. Compared to relative control groups, expressions of HIF-1α protein and VEGF mRNA were significantly higher in D+IR, D+IR+D, and IR+D groups. Conclusion The results indicated that DFO might prevent irradiation-induced hyposalivation by promoting the formation of capillaries.

Key words: salivary gland, radiotherapy, deferoxamine