上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

负载PDGF-BB纳米微球的脱细胞人工真皮修复裸鼠皮肤缺损

刘 诚，付劲松，熊清华，刘淑琴，熊 龙   

  1. 江西省人民医院整形外科， 南昌 330006
  • 出版日期:2014-10-28 发布日期:2014-10-28
  • 作者简介:刘 诚（1967—）， 男， 副主任医师， 硕士; 电子信箱: lc.67@126.com。
  • 基金资助:

    江西省自然科学基金（20122BAB205008）

Application of acellular artificial dermis carrying nanospheres containing platelet derived growth factor-BB for repairing skin defects of nude mice

LIU Cheng, FU Jin-song, XIONG Qing-hua, LIU Shu-qin, XIONG Long   

  1. Department of Plastic Surgery, Jiangxi Provincial People's Hospital, Nanchang 330006, China
  • Online:2014-10-28 Published:2014-10-28
  • Supported by:

    Natural Science Foundation of Jiangxi Province，20122BAB205008

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摘要：

目的 构建负载血小板源性生长因子-BB （PDGF-BB）纳米微球的脱细胞人工真皮，探索将其用于裸鼠皮肤缺损修复的可行性。方法 利用组织工程技术，通过聚乳酸-聚羟基乙酸共聚物（PLGA）包被PDGF-BB制成纳米缓释微球，并黏合在脱细胞人工真皮上，将负载PDGF-BB纳米微球的人工真皮移植至裸鼠动物模型中（实验组，n=10），并以负载空载体纳米微球的人工真皮移植裸鼠作为对照组（n=10），观察携带PDGF-BB纳米微球人工真皮对裸鼠皮肤缺损的修复作用。分别采用Real-Time PCR和Western blotting技术检测局部组织中a-SMA和基膜聚糖（lumican）的表达情况。结果 负载PDGF-BB纳米微球的脱细胞人工真皮较负载空载体纳米微球的人工真皮修复创面时间更短、速率更快，差异有统计学意义（P<0.05）。负载PDGF-BB纳米微球人工真皮移植创面后，局部组织的a-SMA和lumican的相对表达量明显升高。结论 携带PDGF-BB纳米微球脱细胞人工真皮能显著提高皮肤损伤治疗的效果，有可能进一步应用于临床。

关键词: 人工真皮, 生长因子, 纳米微球

Abstract:

Objective To construct the decellular artificial dermis that carries nanospheres containing platelet derived growth factor-BB (PDGF-BB) and to explore the feasibility of repairing skin defects of nude mice by the constructed decellular artificial dermis (DAD). Methods Using tissue engineered technologies, nanospheres were made by enveloping PDGF-BB in the copolymer of polylactic acid-glycolic acid (PLGA). Nanospheres were then bonded to DAD. The decellular human dermis which carried nanospheres containing PDGF-BB was transplanted to nude mice (the experiment group, n=10). Nude mice of the control group (n=10) were transplanted by the decellular human dermis which carried nanospheres not containing PDGF-BB. The repair effect of decellular human dermis which carried nanospheres containing PDGF-BB was observed. Expressions of a-SMA and lumican in local tissue were detected by the Real-Time PCR and Western blotting. Results The decellular human dermis which carried nanospheres containing PDGF-BB repaired skin defects more quickly than the decellular human dermis which carried nanospheres not containing PDGF-BB. The difference was statistically significant (P<0.05). Relative expressions of a-SMA and lumican in local tissue increased significantly after transplantation of the decellular human dermis which carried nanospheres containing PDGF-BB. Conclusion The decellular human dermis which carried nanospheres containing PDGF-BB can significantly improve the treatment of skin defects and is possible to be used in clinical applications.

Key words: artificial dermis, growth factor, nanospheres