上海交通大学学报（医学版）

上海交通大学学报（医学版）

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三氧化二砷对肾母细胞瘤增殖及凋亡的影响

卢立慧，薛天阳，许 伟，高吉照   

  1. 徐州医学院附属医院儿科， 徐州 221000
  • 出版日期:2015-02-28 发布日期:2015-02-27
  • 作者简介:卢立慧（1987—）， 女， 住院医师， 硕士; 电子信箱: lulihui.2007@gmail.com.cn。

Effects of As2O3 on proliferation and apoptosis of SK-NEP-1 cells of human Wilms tumor

LU Li-hui, XUE Tian-yang, XU Wei, GAO Ji-zhao   

  1. Department of Pediatrics, the Hospital Affiliated to Xuzhou Medical College, Xuzhou 221000, China
  • Online:2015-02-28 Published:2015-02-27

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摘要：

目的 观察三氧化二砷（As2O3）对体外人肾母细胞瘤SK-NEP-1细胞株增殖和凋亡的影响。方法 应用0、1.25、2.5、5、10 μmol/L As2O3（A、B、C、D、E组）作用SK-NEP-1细胞24 h。采用MTT比色法检测不同浓度As2O3对SK-NEP-1细胞的增殖抑制率；流式细胞仪检测凋亡率；ELISA法检测SK-NEP-1细胞Bcl-2水平变化；Caspase 3试剂盒检测细胞Caspase 3活性。结果 A、B、C、D、E组细胞增殖抑制率分别为（3.25±1.05）％、（11.19±2.27）％、（20.72±2.21）％、（27.24±0.57）％、（32.25±2.15）％，细胞凋亡率分别为（5.12±0.29）％、（13.42±1.03）％、(23.70±0.97) ％、(32.44±0.82) ％、(40.22±1.31) ％，各组间差异均具有统计学意义 (均P<0.05)。B、C、D、E组Bcl-2蛋白表达水平均低于A组(均P<0.05)， Caspase 3活性均高于A组(均P<0.05)。结论 As2O3能明显抑制肾母细胞瘤细胞的增殖，并进一步诱导其凋亡，其抗肿瘤机制可能与下调Bcl-2表达及激活Caspase 3有关。

关键词: 三氧化二砷, 肾母细胞瘤, 细胞凋亡

Abstract:

Objective To investigate the effects of As2O3 on the proliferation and apoptosis of SK-NEP-1 cells of human Wilms tumor in vitro. Methods The SK-NEP-1 cells of human Wilms tumor was treated with As2O3 of 0, 1.25, 2.5, 5, and 10 μmol/L (group A, B, C, D, and E) for 24 h. The proliferation inhibition rates of different concentrations of As2O3 towards SK-NEP-1 cells were detected by the MTT assay, the apoptosis rates were detected by the flow cytometry; variations of the Bcl-2 level of SK-NEP-1 cells were detected by the ELISA; and the Caspase 3 activity was detected by the Caspase 3 Kit. Results The proliferation inhibition rates of A, B, C, D, and E groups were (3.25±1.05)％, (11.19±2.27)％, (20.72±2.21)％, (27.24±0.57)％, and (32.25±2.15)％, respectively. The apoptosis rates of 5 groups were (5.12±0.29)％, (13.42±1.03)％, (23.70±0.97)％, (32.44±0.82)％, and (40.22±1.31)％, respectively. The differences of 5 groups were statistically significant (P<0.05). The expression level of Bcl-2 of B, C, D, and E groups was lower than that of the A group (P<0.05) and the Caspase 3 activity was higher than that of the A group (P<0.05). Conclusion As2O3 can significantly inhibit the proliferation of SK-NEP-1 cells and induce the apoptosis. The mechanism may be relevant to down-regulating the expression of Bcl-2 and increasing the Caspase 3 activity.

Key words: As2O3, Wilms tumor, cell apoptosis