上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

利用FAP动物模型观察小檗碱对肠道息肉组织中巨噬细胞分型的影响

朴美玉1，曹海龙1，何娜娜1，许梦雀1，鄢芳1，周冰2，王邦茂1   

  1. 1.天津医科大学总医院消化科，  天津 300052； 2.天津市滨海新区塘沽中医医院消化科， 天津 300451
  • 出版日期:2015-03-28 发布日期:2015-03-26
  • 通讯作者: 王邦茂, 电子信箱: gi.tmuh@sohu.com。
  • 作者简介:朴美玉（1980—）， 主治医师， 硕士; 电子信箱: drpiaomy@163.com。
  • 基金资助:

    国家自然科学基金（81070283,81300272）；高等学校博士学科点专项科研基金（20121202110018）；天津市应用基础与前沿技术研究计划（13JCQNJC10600）；天津市滨海新区卫生局科技项目（2013BWKL003）

Effect of berberine on macrophage phenotypes of intestinal polyps using FAP animal models

PIAO Mei-yu1, CAO Hai-long1, HE Na-na1, XU Meng-que1, YAN Fang1, ZHOU Bing2, WANG Bang-mao1   

  1. 1.Department of Gastroenterology, General Hospital, Tianjin Medical University, Tianjin 300052, China； 2.Department of Gastroenterology, Tanggu Traditional Chinese Medicine Hospital of Binhai， Tianjin 300451, China
  • Online:2015-03-28 Published:2015-03-26
  • Supported by:

    National Natural Science Foundation of China, 81070283,81300272; the Specialized Research Fund for the Doctoral Program of Higher Education, 20121202110018; Tianjin Research Program of Application Foundation and Advanced Technology, 13JCQNJC10600; Science and Technology Project of Tianjin Binhai Health Bureau, 2013BWKL003

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摘要：

目的 观察小檗碱对家族性腺瘤性息肉病（FAP）动物模型Apc(Min/+)小鼠肠道息肉组织中巨噬细胞分型的影响，探讨其治疗FAP的作用机制。方法 SPF级4周龄Apc(Min/+)小鼠20只，随机分为小檗碱组和对照组，0.1%的小檗碱混入饮水中给药。连续给药12周后处死小鼠，免疫组织化学法观察息肉组织中巨噬细胞标志物F4/80、M1型巨噬细胞标志物诱导型一氧化氮合酶（iNOS）、M2型巨噬细胞标志物巨噬细胞甘露糖受体（MR）的表达情况；real-time PCR检测iNOS、MR和白介素10（IL-10） mRNA表达。结果 小檗碱组肠道息肉总数较对照组显著降低 (t=16.727，P=0.001)。小檗碱组肠道息肉间质中F4/80表达较对照组明显减少（t=5.327，P=0.043)，iNOS表达较对照组显著增加（t=7.335，P=0.004），而MR表达较对照组明显减少（t=5.634，P=0.016)。小檗碱组iNOS mRNA表达较对照组明显增加(t=7.827，P=0.035)，MR、IL-10 mRNA表达较对照组明显减少(t=6.923，P=0.039；t=8.135，P=0.026)。结论 小檗碱可能通过改变Apc(Min/+)小鼠肠道息肉组织中巨噬细胞的分型，从而发挥抑制息肉生长的作用。

关键词: 小檗碱, Apc(Min/+)小鼠, 肿瘤相关巨噬细胞, 巨噬细胞分型

Abstract:

Objective To observe the effect of berberine on the phenotype of macrophages in the intestinal polyp tissue of Apc(Min/+) mice of familial adenomatous polyposis (FAP) animal model and explore the mechanism of treating FAP. Methods Twenty 4-week-old SPF Apc(Min/+) mice were randomly divided into the control group and berberine group (administrated with berberine of 0.1% in drinking water). Mice were sacrificed after being continuously administrated for 12 weeks. Expressions of macrophage marker F4/80, M1 macrophage marker inducible nitric oxide synthase-2 (iNOS), and M2 macrophage marker macrophage mannose receptor (MR) in the polyp tissue were detected by the immunohistochemistry method. The mRNA expressions of iNOS, MR, and IL-10 were detected by the real-time PCR. Results Compared with the control group, the total number of intestinal polyps of the berberine group was significantly smaller (t=16.727, P=0.001); the expression of F4/80 in stroma of intestinal polyps significantly decreased (t=5.327, P=0.043); the expression of iNOS significantly increased (t=7.335, P=0.004); the expression of MR significantly decreased (t=5.634, P=0.016); the mRNA expression of iNOS significantly increased (t=7.827, P=0.035); and the mRNA expressions of MR and IL-10 significantly decreased (t=6.923, P=0.039; t=8.135, P=0.026). Conclusion Berberine can inhibit the growth of intestinal polyps of Apc(Min/+) mice by altering the phenotype of macrophages in the intestinal polyp tissue.

Key words: berberine, Apc(Min/+) mouse, tumor-associated macrophage, macrophage phenotypes