上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

TET1在子宫内膜病变中的表达及其对癌细胞增殖和迁移能力的影响

谢冰莹1，张箴波1，杨永彬1，丰有吉1，陈晓军2   

  1. 1.上海交通大学附属第一人民医院妇产科， 上海 201620； 2.复旦大学附属妇产科医院， 上海 200011
  • 出版日期:2015-03-28 发布日期:2015-03-26
  • 通讯作者: 丰有吉, 电子信箱: fengyj4806@sohu.com; 陈晓军, 电子信箱: cxjlh@hotmail.com。
  • 作者简介:谢冰莹（1989—）， 女， 蒙古族， 硕士生; 电子信箱: xbyfck@163.com。
  • 基金资助:

    国家自然科学基金（81370688，81370074）

Expression of TET1 in endometrial lesions and its effects on proliferation and migration of endometrial cancer cells

XIE Bing-ying1, ZHANG Zhen-bo1, YANG Yong-bin1, FENG You-ji1, CHEN Xiao-jun2   

  1. 1.Department of Gynecology and Obstetrics, Shanghai First People's Hospital, Shanghai Jiao Tong University, Shanghai 201620, China; 2.Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, China
  • Online:2015-03-28 Published:2015-03-26
  • Supported by:

    National Natural Science Foundation of China, 81370688， 81370074

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摘要：

目的 探讨不同类型子宫内膜病变中TET1的表达水平和干扰TET1蛋白表达对子宫内膜癌细胞系Hec-1a和Ishikawa细胞生物学行为的影响。方法 免疫组织化学检测组织中TET1的表达；应用干扰TET1表达的siRNA转染Hec-1a和Ishikawa细胞，以仅加入转染试剂的细胞作为阴性对照；Western blotting检测转染效率；SRB法检测细胞增殖能力改变；Transwell侵袭试验检测细胞侵袭能力改变；划痕试验检测细胞迁移能力改变；流式细胞术检测细胞周期改变。结果 子宫内膜组织中TET1的表达随病理类型进展而升高；干扰组TET1蛋白表达水平明显低于对照组；干扰TET1表达后子宫内膜癌细胞增殖、迁移和侵袭能力均受到不同程度抑制，同时出现G1/S期细胞周期阻滞。结论 子宫内膜癌及子宫内膜增生性病变组织中TET1表达水平明显高于正常对照组织；干扰TET1表达后子宫内膜癌细胞的增殖、迁移和侵袭能力下降，且出现G1/S期细胞周期阻滞，提示TET1在子宫内膜癌的增殖和转移过程中发挥重要作用。

关键词: 子宫内膜癌, TET1, 增殖

Abstract:

Objective To investigate the expression of ten-eleven translocation 1 (TET1) of different types of endometrial lesions and the effects of intervening the expression of TET1 on biological behaviors of endometrial cancer cell line Hec-1a and Ishikawa cells. Methods The expression of TET1 was detected by the immunohistochemistry. TET1-siRNA was designed, synthesized, and transfected to Hec-1a and Ishikawa cells. Cells with transfection reagent served as negative controls. The efficiency of transfection was detected by the Western blotting. Variations of proliferation, invasion, migration, and cycle of cells were detected by the SRB, Transwell assay, wound scratch assay, and flow cytometry, respectively. Results The expression of TET1 in endometrial tissues increased with the development of pathologic types. The expression of TET1 of the intervention group was significantly lower than that of the control group. The proliferation, invasion, and migration of endometrial cancer cells were more or less inhibited and cell cycle was arrested at G1/S phase after the expression of TET1 was intervened. Conclusion The expression of TET1 in tissue of endometrial hyperplasia and endometrial cancer is significantly higher than that in normal tissue. The proliferation, invasion, and migration of endometrial cancer cells are inhibited and cell cycle is arrested at G1/S phase after the expression of TET1 was intervened, which suggest that TET1 plays an important role in the proliferation and migration of endometrial cancer cells.

Key words: endometrial cancer, TET1, proliferation