上海交通大学学报（医学版）

上海交通大学学报（医学版）

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慢性不完全性睡眠剥夺对幼鼠脂肪细胞因子表达的影响

陈喆1，杨倩1，陈婷1，陈炉1，张泽彧1，严国锋2，戴斐3，郑志杰3，李生慧3   

  1. 上海交通大学1.医学院2010级预防医学班, 2.医学院实验动物科学部, 3.公共卫生学院， 上海 200025
  • 出版日期:2015-04-28 发布日期:2015-04-29
  • 通讯作者: 郑志杰, 电子信箱: zhengzj@sjtu.edu.cn； 李生慧, 电子信箱: lsh9907@163.com。
  • 作者简介:陈喆（1992—）， 男， 本科生; 电子信箱: a1555031@sjtu.edu.cn。
  • 基金资助:

    国家大学生创新性实验计划（201310248080）

Effects of chronic sleep deprivation on expression of adipocytokine of young rats

CHEN Zhe1, YANG Qian1, CHEN Ting1, CHEN Lu1, ZHANG Ze-yu1, YAN Guo-feng2, DAI Fei3, ZHENG Zhi-jie3, LI Sheng-hui3   

  1. 1.2010 Session of Preventive Medicine， Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; 2.Department of Laboratory Animal Science, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China； 3.School of Public Health,Shanghai Jiao Tong University, Shanghai 200025, China
  • Online:2015-04-28 Published:2015-04-29
  • Supported by:

    National University Student Innovation Program， 201310248080

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摘要：

目的 探索慢性不完全性睡眠剥夺对幼鼠循环脂肪细胞因子表达的影响。方法 27只3周龄雄性SD大鼠，随机分为睡眠剥夺组（剥夺组）、睡眠剥夺恢复组（恢复组）、对照组。采用旋转滚轮法建立睡眠剥夺模型。采用酶联免疫吸附法检测血清瘦素、脂联素、视黄醇结合蛋白4 （RBP-4）及内脏脂肪特异性丝氨酸蛋白酶抑制剂（Vaspin）的表达水平。采用李氏指数评价幼鼠体质指数。结果 与对照组相比，剥夺组李氏指数较大（P<0.05）；RBP-4在剥夺组和恢复组的表达水平显著降低（P<0.1）；未发现瘦素、脂联素、Vaspin在各组间具有显著性差异。结论 慢性睡眠剥夺可引起幼鼠肥胖程度增加、RBP-4在血清中的表达水平降低；且随着睡眠的恢复，这些影响没有得到显著改善。

关键词: 幼鼠, 肥胖, 慢性不完全睡眠剥夺, 脂肪细胞因子

Abstract:

Objective To investigate the effects of chronic sleep deprivation on the expression of circulating adipocytokine of young rats. Methods A total of 27 three-week-old Sprague-Dawley (SD) rats were randomly divided into the sleep deprivation group, recovery group, and control group. A motor-driven walking wheel method was adopted to establish the sleep deprivation model. The expressions of serum cytokines, including leptin, adiponectin, RBP-4, and Vaspin, were detected by the ELISA. The LEE's index was used to evaluate the adiposity status of rats. Results The LEE's index of the sleep deprivation group was significantly higher than that of the control group (P<0.05). Compared with the control group, RBP-4 levels of the sleep deprivation group and recovery group significantly decreased (P<0.1). Expressions of leptin, adiponectin, and Vaspin of 3 groups were not significantly different. Conclusion Chronic sleep deprivation results in the increase of adiposity status and decrease of RBP-4 expression in serum, which do not improve significantly with the recovery of sleep.

Key words: young rat, obesity, chronic sleep deprivation, adipocytokine