上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（临床研究） • 上一篇    下一篇

GNA12基因甲基化与子痫前期的相关性分析

沈璐，杨祖菁，叶伟萍，花晓琳   

  1. 上海交通大学 医学院附属新华医院产科， 上海 200092
  • 出版日期:2015-05-28 发布日期:2015-06-04
  • 作者简介:沈璐（1988—）， 女， 硕士生; 电子信箱: lussherry@163.com。
  • 基金资助:

    国家自然科学基金（81401186）

Correlation of methylation of GNA12 and preeclampsia

SHEN Lu, YANG Zu-jing, YE Wei-ping, HUA Xiao-lin   

  1. Department of Obstetrics, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China
  • Online:2015-05-28 Published:2015-06-04
  • Supported by:

    National Natural Science Foundation of China, 81401186

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摘要：

目的 探讨鸟嘌呤核苷酸结合蛋白（GNA12）基因启动子各位点甲基化水平、基因表达与子痫前期之间的关系。方法 选取分娩孕妇中子痫前期患者（病例组）和正常孕妇（对照组）胎盘各50例，外周血各25例，采用甲基化特异性PCR技术和real-time PCR技术分析GNA12基因启动子区CpG岛甲基化状态和mRNA的表达。结果 病例组的胎盘组织及孕母外周血的GNA12 63位点的甲基化程度均低于对照组（P=0.003，P=0.004 3）；病例组mRNA表达量高于对照组(P=0.013)；胎盘组织甲基化率与外周血甲基化率具有相关性（P<0.000 1）。结论 GNA12基因启动子的甲基化水平与子痫前期的发病存在相关性。通过检测孕母外周血GNA12基因，可能可预测子痫前期的发生。

关键词: 子痫前期, 表观遗传学, 鸟嘌呤核苷酸结合蛋白, DNA甲基化

Abstract:

Objective To investigate the relationship of methylation level and gene expression of gene promoter of guanine nucleotide binding protein (GNA12) and the preeclampsia. Methods A total of 50 patients with preeclampsia (preeclampsia group) and 50 normal pregnant women (control group) were selected. From each group, 50 placenta tissue samples and 25 peripheral blood samples were collected. The methylation-specific PCR and real-time fluorescence quantitative PCR were adopted to analyze the methylation and mRNA expression of CpG island of gene promoter region of GNA12. Results The degree of methylation of locus 63 of GNA12 in placenta tissue and peripheral blood of the preeclampsia group were lower than those of the control group (P=0.003，P=0.004 3). The mRNA expression of the preeclampsia group was higher than that of the control group (P=0.013). The methylation rate of placenta tissues correlated with the methylation rate of peripheral blood (P<0.000 1). Conclusion The methylation of gene Promoter of GNA12 correlates with the incidence of preeclampsia. The incidence of preeclampsia may be predicted by detecting gene GNA12 in peripheral blood of pregnant women.

Key words: preeclampsia, epigenetics, guanine nucleotide binding protein, methylation