上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

NK细胞与NKDCs的相关性及功能研究

傅于捷1，高建新2，李林凤2，赵晓菁1   

  1. 上海交通大学 医学院附属仁济医院 1.胸外科， 2.干细胞研究中心， 上海 200127
  • 出版日期:2016-06-28 发布日期:2016-07-25
  • 通讯作者: 赵晓菁, 电子信箱: zhaoxiaojing@renji.com。
  • 作者简介:傅于捷（1976—）， 男， 主治医师， 博士生; 电子信箱: richardjiejie@hotmail.com。
  • 基金资助:

    国家自然科学基金（81402287）

Correlation and function of NK cells and NKDCs

FU Yu-jie1, GAO Jian-xin2, LI Lin-feng2, ZHAO Xiao-jing1   

  1. 1.Department of Thoracic Surgery, 2.Stem Cell Research Center, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China
  • Online:2016-06-28 Published:2016-07-25
  • Supported by:

    National Natural Science Foundation of China， 81402287

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摘要：

目的 研究自然杀伤细胞(NK细胞)与自然杀伤树突状细胞（NKDCs）在免疫系统重建中的变化及相互关系。方法 利用骨髓重建小鼠模型，采用流式细胞仪分选NK细胞、树突状细胞（DCs）和NKDCs；免疫佐剂CpG体外刺激NK细胞和NKDCs，检测细胞因子IFN-γ的分泌情况。结果 在免疫系统重建过程中，骨髓或脾脏的NK细胞和NKDCs均在1周内出现升高，至第4周恢复到正常水平，提示天然免疫功能在骨髓重建过程中首先重建；其次，NK细胞和NKDCs在功能上是两群性质不同的细胞，NKDCs产生IFN-γ的能力高于NK细胞。CpG可以刺激NKDCs产生IFN-γ，但对NK细胞无相应作用。INF-γ的产生与NK细胞或NKDCs表达B220水平无关。结论 NKDCs可能在天然抗肿瘤免疫中起关键作用。

关键词: 自发性抗肿瘤免疫, 树突状细胞, 自然杀伤细胞, 自然杀伤树突状细胞

Abstract:

Objective To investigate the changes in natural killer (NK) cells and natural killer dendritic cells (NKDCs) during immune system rebuilding, and explore the correlation between NKDCs and NK cells. Methods A mouse model of bone marrow reconstruction was used, and NK cells, dendritic cells (DCs) and NKDCs were sorted with flow cytometry. NK cells and NKDCs were stimulated with the immunologic adjuvant CpG in vitro, and the secretion of cytokine IFN-γ was detected. Results In the process of rebuilding the immune system, the numbers of both NK cells and NKDCs in bone marrow or spleen increased within one week, and returned to normal at week 4, suggesting that innate immune function was rebuilt first in bone marrow rebuilding. NK cells and NKDCs were functionally different. NKDCs produced more IFN-γ as compared with NK cells. CpG stimulated NKDCs to produce IFN-γ, but it was not the case for NK cells. INF-γ production was not associated with B220 expression in NK cells or NKDCs. Conclusion NKDCs may play a key role in the innate anti-tumor immunity.

Key words: natural occurring anti-tumor immunity, dendritic cells, natural killer cells; , natural killer dendritic cells