上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2018, Vol. 38 ›› Issue (2): 174-.doi: 10.3969/j.issn.1674-8115.2018.02.011

• 论著（临床研究） • 上一篇    下一篇

不同对氧磷酶 1 基因型孕妇有机磷农药暴露与氧化应激水平的相关性

季麟 1 ，茹鲜古丽·艾姆孜 1, 2，张妍 1 ，施蓉 1 ，周义军 1 ，成晓蒙 3 ，王雪梅 3 ，田英 1 ，高宇 1   

  1. 1. 上海交通大学 公共卫生学院，上海 200025；2. 上海交通大学 医学院附属新华医院环境与儿童健康重点实验室，上海 200092；3. 山东省潍坊市滨海经济技术开发区大家洼街道社区卫生服务中心，潍坊 262737
  • 出版日期:2018-02-28 发布日期:2018-03-09
  • 通讯作者: ?高 宇，电子信箱：gaoyu_ciel@sjtu.edu.cn。
  • 基金资助:
    国家自然科学基金青年基金（81602823）；上海市卫生和计划生育委员会面上项目（201640174）；上海交通大学医学院附属新华医院“千天计划”（15QT08）；上海卫生计生系统重要薄弱学科建设计划（2016ZB0103）

Association between exposure to organophosphate pesticides and levels of oxidative stress in pregnant women with different paraoxonase 1 genotypes

JI Lin1 , RUXIANGULI Aimuzi1, 2, ZHANG Yan1 , SHI Rong1 , ZHOU Yi-jun1 , CHENG Xiao-meng3 , WANG Xue-mei3 , TIAN Ying1 , GAO Yu1   

  1. 1. School of Public Health, Shanghai Jiao Tong University, Shanghai 200025, China; 2. MOE and Shanghai Key Laboratory of Children’s Environmental Health, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China; 3. Community Health Service Center of Dajiawa Lane, Binhai Economic and Technical Development District, Weifang, Shandong Province, Weifang 262737, China
  • Online:2018-02-28 Published:2018-03-09
  • Supported by:
    National Natural Science Foundation of China, 81602823; Scientific Research Program  of Shanghai Municipal Commission of Health and Family Planning, 201640174; Early Life Plan of Xinhua Hospital, Shanghai Jiao Tong University School of Medicine,  15QT08; Developing Plan of Important Weak Disciplines from Shanghai Municipal Commission of Health and Family Planning, 2016ZB0103

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摘要： 目的 · 结合对氧磷酶 1（paraoxonase 1，PON1）基因型探讨孕妇有机磷农药（organophosphate pesticides，OPs）暴露水平与体内
氧化应激水平之间的关系。方法 · 以山东省某医院的 204 名孕晚期妇女为研究对象，检测尿液中 4 种 OPs 非特异性二烷基磷酸酯代谢
产物水平，测定血清中的氧化应激标志物 [ 总自由巯基（-SH）、丙二醛（MDA）] 水平，并提取全血中的 DNA 检测 PON1-108、PON1192、
PON155 位点的基因型。采用多元线性回归模型分析全人群及不同 PON1 基因型的个体中 OPs 暴露水平与氧化应激标志物水平之间的关
联。结果 · 未见 OPs 暴露与氧化应激之间存在显著关联。但根据 PON1 基因型分层后发现在 PON1192RR 基因型的孕妇中，总磷酸二甲酯类
水平 [β（95% CI）：-104.10（-191.31，-16.88）] 及总二烷基磷酸酯类水平 [β（95% CI）：-111.78（-221.84，-1.72）] 与 -SH 水平呈负相
关，而在其他基因型的个体中未发现这一关联。结论 · OPs 暴露可能与 PON1192RR 基因型的孕妇体内氧化应激水平升高有关。

关键词: 氧化应激, 有机磷农药, 对氧磷酶 1, 孕妇

Abstract:

 To investigate whether paraoxonase 1 (PON1) genotypes were effect modifiers in the relationship between exposure to organophosphate pesticides (OPs) and oxidative stress level in pregnant women. Methods · A total of 204 pregnant women recruited from a hospital in Shandong Province were included in the study. Four nonspecific dialkyl phosphate (DAP) metabolites of OPs were measured in each urine sample. Levels of two oxidative stress biomarkers [total free sulfhydryl (-SH) and malondialdehyde (MDA)] were measured in serum samples. Blood samples were also analyzed for detecting PON1 genotypes (PON1-108, PON1192 and PON155). Separate linear regression models were conducted to explore the relationship between DAP metabolite levels and oxidative stress levels in all 204 pregnant women or women within each PON1 genotype. Results · There was no significant association between DAP metabolite levels and oxidative stress levels in all 204 women. Levels of dimethyl phosphates [β (95% CI): -104.10 (-191.31, -16.88)] and dialkyl phosphates [β (95% CI): -111.78 (-221.84, -1.72)] were negatively associated with -SH level among pregnant women with PON1192RR genotype, but this association was not found among women with other genotypes. Conclusion · OP exposure may be associated with a higher oxidative stress level among pregnant women with PON1192RR genotype.

Key words: oxidative stress, organophosphate pesticides, paraoxonase 1, pregnant women