上海交通大学学报(医学版) ›› 2019, Vol. 39 ›› Issue (1): 21-.doi: 10.3969/j.issn.1674-8115.2019.01.005

• 论著·基础研究 • 上一篇    下一篇

内质网应激通路需肌醇酶 1 α/X盒结合蛋白 1在中性粒细胞弹力蛋白酶诱导的气道黏液分泌中的作用

李琪 1,周向东 1,曾曼 1,Victor P. KOLOSOV2,Juliy M. PERELMAN2   

  1. 1. 海南医学院第一附属医院呼吸内科,海口 570102;2. 俄罗斯医学科学院远东呼吸生理与病理研究中心,布拉戈维申斯克 675000
  • 出版日期:2019-01-28 发布日期:2019-02-27
  • 通讯作者: 周向东,电子信箱:zxd999@263.net。
  • 作者简介:李琪(1982—),女,副主任医师,博士;电子信箱:lqlq198210@sina.com.
  • 基金资助:
    国家自然科学基金地区科学基金项目( 31660329,81660010,81860001);国家自然科学基金中俄合作项目( 81811530063,81811530115);海南省自然科学基金( 817322);海南省科协青年科技英才创新计划项目( QCXM201702)

Role of inositol-requiring kinase 1α/X-box binding protein 1 in airway mucus secretion inducedneutrophil elastase

LI Qi1, ZHOU Xiang-dong1, ZENG Man1, Victor P. KOLOSOV2, Juliy M. PERELMAN2   

  1. 1. Division of Respiratory Medicine, First Affiliated Hospital, Hainan Medical College, Haikou 570102, China; 2. Far Eastern Scientific Center of Physiology and Pathology of Respiration, Siberian Branch, Russian Academy of Medical Sciences, Blagoveschensk 675000, Russia
  • Online:2019-01-28 Published:2019-02-27
  • Supported by:
    Regional Scientific Project of National Natural Science Foundation of China, 31660329, 81660010, 81860001;NSFC-RFBR Cooperation Project, 81811530063, 81811530115; Natural Science Foundation of Hainan Province, 817322; Innovation Project of Hainan Science and Technology Association for Talent Youth, QCXM201702

摘要: 目的 ·探讨内质网应激( endoplasmic reticulum stress,ERS)反应在中性粒细胞弹力蛋白酶( neutrophil elastase,NE)诱导人气道上皮细胞黏蛋白 5AC(mucin 5AC,MUC5AC)分泌中的作用及机制。方法 ·培养人气道上皮细胞 HBE16,分别给予活性氧(reactive oxygen species,ROS)抑制剂 N-乙酰半胱氨酸( N-acetylcysteine,NAC)、ERS抑制剂 4-苯基丁酸( 4-phenylbutyrate, 4-PBA)预处理,或分别转染需肌醇酶 1α(inositol-requiring kinase 1α,IRE-1α)小干扰 RNA(siRNA)、X盒结合蛋白 1(X-box binding protein 1,XBP-1)siRNA,再予以 NE刺激;同时设单纯 NE组和空白对照组。试剂盒检测 ROS的生成水平; Western blotting检测干预后 ERS相关信号分子葡萄糖调节蛋白 78(glucose-regulated protein 78,GRP78)、磷酸化蛋白激酶 R样内质网激酶(phosphorylated protein kinase R-like endoplasmic reticulum kinase,pPERK)、活化转录因子 6(activating transcription factor 6,ATF6)、磷酸化 IRE-1α(pIRE-1α)及 XBP-1蛋白表达量的变化;实时荧光定量 PCR检测剪切的 XBP-1(XBP-1s)mRNA水平; ELISA和免疫荧光检测 MUC5AC在各组细胞中的蛋白表达情况。结果 ·与空白对照组相比, NE刺激 24 h后细胞 ROS含量增加, GRP78、 ATF6、pPERK及 pIRE-1α蛋白水平均显著升高(均 P<0.05),XBP-1s mRNA及蛋白表达也明显增加(均 P<0.05),同时 MUC5AC蛋白表达增加(均 P<0.05);NAC及 4-PBA预处理后均使上述 ERS相关蛋白表达较单纯 NE组降低(均 P<0.05),MUC5AC蛋白水平降低(均 P<0.05);转染 IRE-1α siRNA或 XBP-1 siRNA,细胞中 MUC5AC蛋白表达也较单纯 NE组明显降低,同时 XBP-1s mRNA及蛋白表达也有明显下降(均 P<0.05)。结论 · NE通过产生 ROS引起 ERS反应,诱导 MUC5AC蛋白表达及分泌增加, ERS通路 IRE-1α/XBP-1在其中发挥着一定的作用。

关键词: 内质网应激, 需肌醇酶1&, alpha, X盒结合蛋白1, 中性粒细胞弹力蛋白酶, 活性氧, 黏蛋白5AC

Abstract:

Objective · To explore the effect of endoplasmic reticulum stress (ERS) on neutrophil elastase (NE) induced mucin 5AC (MUC5AC) production in human airway epithelial cells. Methods · HBE16 airway epithelial cells were cultured and pretreated with reactive oxygen species (ROS) inhibitor N-acetylcysteine (NAC) or ERS inhibitor 4-phenylbutyrate (4-PBA), or transfected with small interfering RNA (siRNA) against inositolrequiring kinase 1α (IRE-1α) or X-box binding protein 1 (XBP-1), respectively before incubation with NE. NE group and blank control group were also set up. ROS production was assayeddetection kit; of glucose-regulated protein 78 (GRP78), phosphorylated protein kinase R-like endoplasmic reticulum kinase (pPERK), activating transcription factor 6 (ATF6), phosphorylated IRE-1α (pIRE-1α), and XBP-1 protein was detectedWestern blotting; spliced XBP-1 (XBP-1s) mRNA was measuredreal-time PCR; levels of MUC5AC protein in culture supernatant and cytoplasm were assayedELISA and immunofluorescence. Results · There was an obvious increase of ROS production with strong elevation of GRP78, ATF6, pPERK, and pIRE-1α protein in NE group cells after 24 h, compared with blank control group (P<0.05). The protein and mRNA of XBP-1s, and MUC5AC production also increased obviously (P<0.05). NAC and 4-PBA reduced ERS-related protein and MUC5AC production and secretion (P<0.05). Further studies showed that MUC5AC secretion was also bluntedIRE-1α siRNA or XBP-1 siRNA, accompanied with decreased of XBP-1s mRNA and protein (P<0.05). Conclusion · NE induces ERSproducing ROS, and increases MUC5AC protein production and secretion; IRE-1α/XBP-1 play a certain role in this process.

Key words: endoplasmic reticulum stress (ERS), inositol-requiring kinase 1&, alpha, (IRE-1&, alpha, ), X-box binding protein 1 (XBP-1), neutrophil elastase (NE), reactive oxygen species (ROS), mucin 5AC (MUC5AC)

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