上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2019, Vol. 39 ›› Issue (1): 16-.doi: 10.3969/j.issn.1674-8115.2019.01.004

• 论著·基础研究 • 上一篇    下一篇

小鼠巨噬细胞对钩端螺旋体 56606v和 56606a的吞噬及炎症应答的比较研究

范霞 1，夏碧丽 2，吕霖 1，徐梦莎 3，李佳茵 1，何平 1   

  1. 1. 上海交通大学基础医学院免疫学与微生物学系，上海 200025；2. 复旦大学附属公共卫生临床中心，上海 201508；3. 浙江省台州恩泽医疗集团恩泽医院药剂科，台州 318050
  • 出版日期:2019-01-28 发布日期:2019-02-27
  • 通讯作者: 何平，电子信箱：hpatsh@sjtu.edu.cn。
  • 作者简介:范霞（1993—），女，硕士生；电子信箱：fanx_fx@163.com。
  • 基金资助:
    国家自然科学基金面上项目（81471908）

Comparison of the phagocytosis and inflammatory response of murine macrophages infectedLeptospirastrains 56606v and 56606a

FAN Xia1, XIA Bi-li2, Lü Lin1, XU Meng-sha3, LI Jia-yin1, HE Ping1   

  1. 1. Department of Immunology and Microbiology, Shanghai Jiao Tong University College of Basic Medical Sciences, Shanghai 200025, China; 2. Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China; 3. Taizhou Enze Medical Center, Zhejiang Province, Taizhou 318050, China
  • Online:2019-01-28 Published:2019-02-27
  • Supported by:
    National Natural Science Foundation of China, 81471908

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摘要： 目的 ·比较问号钩端螺旋体 56606v株和 56606a株对小鼠巨噬细胞的感染情况，探究致病性钩端螺旋体的致病机制。方法 ·用问号钩端螺旋体强毒株 56606v株和减毒株 56606a株分别感染 C57BL/6小鼠腹腔巨噬细胞后，对样本进行免疫荧光染色，观察巨噬细胞对钩端螺旋体的吞噬及清除能力，并通过 realtime-PCR检测感染后巨噬细胞的细胞因子的表达。结果 ·免疫荧光染色和 realtime-PCR结果均发现在感染 72 h后，56606v株仍能在巨噬细胞中存活，而 56606a株不能在巨噬细胞中存活；巨噬细胞对 56606v株的吞噬率高于对 56606a株的吞噬率，而感染 56606v株后细胞因子的表达低于感染 56606a株后的水平。结论 ·问号钩端螺旋体强毒株 56606v株能在巨噬细胞中存活，并引起较低的炎症反应，可借此逃避免疫细胞的杀伤从而引发疾病；而减毒株 56606a株被巨噬细胞吞噬后则被清除，且引发较强的炎症反应，易被机体免疫细胞清除，从而不引起疾病。

关键词: 钩端螺旋体, 巨噬细胞, 吞噬, 炎症反应, 免疫逃逸

Abstract:

Objective · To compare the response and consequence of momacrophages infectedLeptospira interrogans 56606v and 56606a, and explore the mechanisms of pathogenic Leptospira to cadisease. Methods · Peritoneal macrophages of C57BL/6 mice were infectedpathogenic Leptospira 56606v and non-pathogenic Leptospira 56606a respectively. Immunofluorescence staining was performed to observe phagocytosis and clearance of Leptospira after infection, and realtime-PCR was used to determine cytokine production of macrophages. Results · After 72-hour infection, strain 56606v exhibited a lower phagocytic rate but survived after incubation with peritoneal macrophages compared with strain 56606a, which showed a higher phagocytic rate but was cleared at that time point. Additionally, cytokine production of macrophages incubated with 56606v was lower than that with 56606a. Conclusion · Leptospira interrogans strain 56606v can survive in macrophages, which may contribute to evasion phagocytic clearance and lead to disease, while strain 56606a can be cleared, which implicates a lower pathogenicity.

Key words: Leptospira interrogans, macrophage, phagocytosis, inflammatory response, immune surveillance

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