上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2019, Vol. 39 ›› Issue (12): 1382-.doi: 10.3969/j.issn.1674-8115.2019.12.007

• 论著·基础研究 • 上一篇    下一篇

脂肪干细胞对转化生长因子β1干预下真皮成纤维细胞致纤维化能力的同步影响

史永平1，原 博2   

  1. 1.浙江省嘉兴市第一医院烧伤整形科，嘉兴314000；2.上海交通大学医学院附属瑞金医院烧伤整形科，上海 200025
  • 出版日期:2019-12-28 发布日期:2020-02-06
  • 通讯作者: 原 博，电子信箱：hiyuanbo2002@163.com。
  • 作者简介:史永平（1982—），男，主治医师，硕士；电子信箱：344694646@qq.com。
  • 基金资助:
    国家自然科学基金（81101433）

Real-time effect of adipose stem cells on fibrogenesis of dermal fibroblast co-stimulatedtransforming growth factor-β1

SHI Yong-ping, YUAN Bo   

  1. 1. Department of Burns and Plastic Surgery, the First Hospital of Jiaxing, Zhejiang Province, Jiaxing 314000, China; 2. Department of Burns and Plastic Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
  • Online:2019-12-28 Published:2020-02-06
  • Supported by:
    National Natural Science Foundation of China, 81101433

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摘要： 目的·进一步明确脂肪干细胞在创面修复中的可能作用和内在机制，探讨脂肪干细胞对真皮成纤维细胞致纤维化能力的同步影响。方法·通过Transwell和接触共培养2种实时共培养模式，观察真皮成纤维细胞在有或无脂肪干细胞和/或转化生长因子β1（transforming growth factor-β1，TGF-β1）作用72 h后致纤维化能力的变化，包括真皮成纤维细胞数量的变化；用细胞免疫荧光和免疫印迹法检测Ⅰ型、Ⅲ型胶原表达情况。结果·Transwell体系中，脂肪干细胞与TGF-β1共同作用下的成纤维细胞数量较单一TGF-β1作用组明显减少(P0.035)；接触共培养体系中，在TGF-β1作用下，脂肪干细胞“基底细胞”组与成纤维细胞“基底细胞”组比较，荧光标记成纤维细胞的数量显著减少（P0.000）。在Transwell 体系中，脂肪干细胞与成纤维细胞共培养时，上室中成纤维细胞分泌胶原量明显增加（P0.000）；接触共培养体系中，在TGF-β1作用下，脂肪干细胞“基底细胞”组与成纤维细胞“基底细胞”组比较，培养液上清中胶原分泌量明显升高（P0.000）。结论·脂肪干细胞可能通过旁分泌或直接接触途径对TGF-β1干预作用下的真皮成纤维细胞致纤维化能力产生同步影响，既能促进胶原合成和分泌，同时又能抑制成纤维细胞的过度增殖。

关键词: 脂肪干细胞, 成纤维细胞, 转化生长因子&, beta, 1, 增殖, 胶原

Abstract:

Objective · To mainly explore the real-time effect of adipose stem cells (ASCs) on the fibrogenesis of dermal fibroblasts co-stimulatedtransforming growth factor-β1 (TGF-β1), and further clarify the possible pathway and mechanism of ASCs in regulating wound repair. Methods ·using two different real-time culture systems including Transwell system and contact co-culture system, events associated with fibrogenesis including the changes of fibroblast cell number or of collagen types Ⅰ and Ⅲ detectedimmunofluorescence or Western blotting in dermal fibroblasts at 72 h with/without the stimulation of transforming growth factor-β1 (TGF-β1) and/or ASCs were studied. Results · In Transwell system, the cell number of fibroblasts was significantly decreased under the stimulation of ASCs and TGF-β1, compared with TGF-β1 only group (P0.035). In contact co-culture system, under the stimulation of TGF-β1, the numbers of fluorescence labeling fibroblasts in group with ASCs as basal cells were decreased, compared with group with fibroblast as basal cells (P0.000). In terms of the collagen , in Transwell system, the amounts of collagen secretion fibroblasts within the upper chamber were increased dramatically when fibroblasts were being co-cultured with ASCs (P0.000). In contact co-culture system, under the stimulation of TGF-β1, the amounts of collagen secretion in the supernatant of cell culture in the group with ASCs as basal cells were increased, compared with the group with fibroblast as basal cells (P0.000). Conclusion · ASCs may have an effect on fibrogenesis of dermal fibroblasts co-stimulatedTGF-β1 through a paracrine and direct contact way. It not only increases collagen production and secretion, but also inhibits fibroblasts over-proliferation.

Key words: adipose stem cell, fibroblasts, transforming growth factor-β1, proliferation, collagen