上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2019, Vol. 39 ›› Issue (12): 1389-.doi: 10.3969/j.issn.1674-8115.2019.12.008

• 论著·基础研究 • 上一篇    下一篇

耐药肺炎克雷伯菌噬菌体JD902的分离及生物学特性和安全性研究

吕 霖，石 鑫，郭晓奎，秦金红   

  1. 上海交通大学基础医学院免疫学与微生物学系，上海 200025
  • 出版日期:2019-12-28 发布日期:2020-02-06
  • 作者简介:吕 霖（1994—），男，硕士生；电子信箱：764874087@qq.com。
  • 基金资助:
    上海市自然科学基金（17ZR1415900）

Isolation, biological characteristics and genomic safety assessment of drug-resistant Klebsiella pneumoniae phage JD902

Lü Lin, SHI Xin, GUO Xiao-kui, QIN Jin-hong   

  1. Department of Microbiology and Immunology, Shanghai Jiao Tong University College of Basic Medical Sciences, Shanghai 200025, China
  • Online:2019-12-28 Published:2020-02-06
  • Supported by:
    Natural Science Foundation of Shanghai, 17ZR1415900

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902

摘要： 目的·筛选耐药肺炎克雷伯菌的噬菌体，并检验其是否符合耐药肺炎克雷伯菌噬菌体库的入库要求。方法·将分离自1例泌尿系统感染患者的1株耐药肺炎克雷伯菌作为宿主菌，从环境污水中进行噬菌体筛选，分离、纯化噬菌体并进行生物学特性的研究及噬菌体基因组测序，并基于全基因组数据分析其使用安全性。结果·分离到1株耐药肺炎克雷伯菌噬菌体，将其命名为JD902，属有尾目，裂解量为88 pfu/cell，在pH值4.0～11.0范围内都能保持较好的活性。基因组分析表明，其不携带抗生素抗性基因和毒力因子基因。结论·JD902为1株烈性噬菌体，体外稳定性及安全性均较高，可以纳入临床治疗使用噬菌体库。

关键词: 肺炎克雷伯菌, 噬菌体, 抗生素耐药性, 生物学特性, 安全性评估

Abstract:

Objective · To screen for a bacteriophage of a drug-resistant Klebsiella pneumoniae strain and assess its eligibility to be incorporated into the Klebsiella bacteriophage library. Methods · A drug-resistant Klebsiella pneumoniae strain isolated a patient with urinary infection was used as the host strain. Phage screening was carried out in environmental sewage, and the bacteriophage was isolated, purified, characterized and sequenced. Safety evaluation was also conducted based on the genome sequence. Results · A drug-resistant Klebsiella pneumoniae bacteriophage was isolated and designated as JD902, which belonged to Caudovirales. The burst size was 88 pfu/cell, and it could maintain good activity in pH range 4.0-11.0. Genomic analysis demonstrated that it did not carry any antibiotic resistance genes and virulence factor genes. Conclusion · JD902 is a virulent phage with high stability and safety in vitro. It can be incorporated into bacteriophage library for clinical treatment.

Key words: Klebsiella pneumoniae, bacteriophage, antibiotic resistance, biological characteristic, safety evaluation