上海交通大学学报（医学版）

上海交通大学学报(医学版) ›› 2020, Vol. 40 ›› Issue (10): 1365-1370.doi: 10.3969/j.issn.1674-8115.2020.10.011

• 论著·基础研究 • 上一篇    下一篇

浓缩生长因子与血凝块诱导根管内组织再生的对比研究

张诗韵，赖光云，汪 俊   

  1. 上海交通大学医学院附属第九人民医院·口腔医学院儿童口腔科，国家口腔疾病临床医学研究中心，上海市口腔医学重点实验室，上海市口腔医学研究所，上海 200011
  • 出版日期:2020-10-28 发布日期:2020-11-27
  • 通讯作者: 汪 俊，电子信箱：wangjun202@126.com。
  • 作者简介:张诗韵（1993—），女，住院医师，硕士；电子信箱：zsy_0211@163.com。
  • 基金资助:
    上海市卫生健康委员会卫生行业临床研究专项（20194Y0049）；上海交通大学医学院附属第九人民医院临床研究助推计划（JYLJ010）；上海交通大学医学院附属第九人民医院生物样本库项目（YBKB201903）。

Comparative study on the regeneration capacity of tissue in root canals between concentrated growth factor and blood clot

ZHANG Shi-yun, LAI Guang-yun, WANG Jun   

  1. Department of Pediatric Dentistry, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai 200011, China
  • Online:2020-10-28 Published:2020-11-27
  • Supported by:
    Clinical Research Program of Health Care Industry of Shanghai Municipal Health Commission (20194Y0049); Clinical Research Program of Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine (JYLJ010); Biological Sample Bank Project of Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine (YBKB201903).

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摘要： 目的·通过动物实验比较浓缩生长因子（concentrated growth factor，CGF）与血凝块诱导根管内组织再生的差异。方法·取健康志愿者上臂静脉血，制备CGF。将CGF植入离体牙牙根片段，作为实验组；将全血注入离体牙牙根片段后形成血凝块，作为对照组，每组18个样本。分别在裸鼠两侧背部皮下注入对照组样本和实验组样本，并于移植4周及6周后取出。采用苏木精-伊红染色（hematoxylin and eosin staining，H-E staining，H-E染色）观察根管内新生组织的结构并计算其面积占比。采用免疫组织化学分析、免疫荧光分析及改良Masson染色检测新生组织成分，并计算各成分的面积占比和微血管密度。结果·2组诱导产生的新生组织结构类似，其主要成分为胶原纤维、血管、神经纤维和脂肪组织。移植4周和6周后，实验组新生组织面积占比均高于对照组（P=0.026，P=0.040），其微血管密度亦均高于对照组（P=0.012，P=0.022）。移植6周后，与对照组相比，实验组新生神经纤维面积占比增加（P=0.047）。结论·CGF与血凝块诱导的根管内组织再生的能力类似，其或可为牙髓再生治疗提供新的选择。

关键词: 浓缩生长因子, 血小板浓缩物, 牙髓再生治疗, 细胞归巢

Abstract:

Objective · To compare the difference of the regeneration capacity of tissue in root canals between concentrated growth factor (CGF) and blood clot by animal study. Methods · CGF was prepared from the upper arm venous blood of healthy volunteers. CGF was implanted into the root segments of isolated teeth, which is the experimental group, and the whole blood was injected into the root segments to form blood clots, which is the control group, with 18 samples in each group. The samples of the control group and experimental group were injected subcutaneously on both sides of the back of nude mice, respectively, and were taken out 4 and 6 weeks after transplantation. Hematoxylin and eosin staining (H-E staining) was used to observe the structure of newly formed tissue in root canals and calculate its area proportion. Immunohistochemical analysis, immunofluorescence analysis and modified Masson staining were used to detect the components of the newly formed tissue, and the area ratio and microvessel density of each component were calculated. Results · The structure of the newly formed tissue induced by the two groups was similar, and the main components were collagen fibers, blood vessels, nerve fibers and adipose tissue. After 4 and 6 weeks of implantation, compared with the control group, the proportion of the newly formed tissue area and microvessel density in the experimental group were higher (all P<0.05). After 6 weeks of implantation, compared with the control group, the proportion of new nerve fibers in the experimental group was increased (P=0.047). Conclusion · CGF and blood clot-induced endodontic tissue regeneration ability is similar, which may imply that CGF can be an alternative to blood clot in the regenerative endodontic treatment.

Key words: concentrated growth factor (CGF), platelet concentrate, regenerative endodontic treatment, cell homing

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