弥漫内生性脑桥胶质瘤靶向治疗新策略的体外筛选与验证

doi:10.3969/j.issn.1674-8115.2021.08.001

摘要/Abstract

摘要：

目的·从表观遗传角度寻找和鉴定弥漫内生性脑桥胶质瘤（diffuse intrinsic pontine glioma，DIPG）的单药治疗和组合靶向治疗新策略。方法·以已发表的基于8例DIPG肿瘤组织和6例正常脑组织的转录组数据为基础选择用于筛选实验的靶向小分子库。在DIPG原代细胞SU_DIPG13中进行单药筛选并寻找新的能显著抑制DIPG细胞生长的靶向小分子。通过实时定量PCR和Western blotting检测药物处理后其靶基因在mRNA和蛋白水平的表达变化。EdU和Annexin V/碘化丙啶染色后利用流式细胞术分别检测药物处理对DIPG原代细胞增殖和凋亡的影响。靶向小分子库中的药物分别与溴结构域和外端家族（bromodomain and extra terminal protein，BET）抑制剂JQ1和panobinostat组蛋白去乙酰化酶（histone deacetylase，HDAC）进行组合筛选，并体外验证对DIPG存在抑制作用的药物组合。结果·选择了包含66个小分子靶向小分子的药物库用于单药和组合筛选。单药筛选鉴定出的YM155能够显著抑制DIPG原代细胞SU_DIPG13和SU_DIPG17的生长，其靶基因BIRC5（baculoviral IAP repeat containing 5；编码survivin）在DIPG肿瘤组织中的表达高于正常组织（P=0.018）。YM155能抑制BIRC5在mRNA和蛋白水平的表达。YM155既能抑制DIPG原代细胞的增殖又能促进其凋亡。靶向小分子库中的CX4945、ABT-737分别与JQ1、panobinostat联用能在体外协同抑制DIPG细胞活性。结论·通过单药和组合药物筛选鉴定出了DIPG的靶向治疗新策略，为后续体内验证这些DIPG的新靶向治疗策略和挖掘治疗机制奠定了基础。

关键词: 弥漫内生性脑桥胶质瘤, 靶向小分子单药筛选, YM155, BIRC5基因, 药物组合筛选

Abstract:

Objective·To find and identify new targeted therapeutic compounds and combinations for diffuse intrinsic pontine glioma (DIPG) from the perspective of epigenetics.

Methods·Selection of small molecule compounds was based on the previously published transcriptome analysis of 8 cases of DIPG and 6 cases of normal brain tissues. New inhibitory compounds of DIPG were identified by single agent screening in DIPG primary tumor cells. The changes of target genes at mRNA and protein expression level were detected by real-time PCR and Western blotting after drug treatment. The effects of drug treatment on the proliferation and apoptosis of DIPG primary tumor cells were detected by FACS analyses after EdU and Annexin V/propidium iodide staining, respectively. The combinatory screening of small molecular compounds was performed with bromodomain and extra terminal protein (BET) inhibitor JQ1 or histone deacetylase (HDAC) inhibitor panobinostat, and the drug combination with inhibitory effect on DIPG was verified in vitro.

Results·Sixty-six small molecules were chosen to be applied to screening. Single agent screening identified that YM155 could significantly inhibit DIPG primary tumor cell growth, and BIRC5 (baculoviral IAP repeat containing 5; gene encoding survivin), a target gene of YM155, was significantly upregulated in DIPG tumor tissues (P=0.018). YM155 could reduce the expression of BICR5 at both mRNA and protein levels. YM155 could repress proliferation and induce apoptosis of DIPG. CX4945 and ABT-737 from the targeted small molecular library were combined with JQ1 (BET inhibitor) and panobinostat (HDAC inhibitor), respectively,which could synergistically inhibit the activity of DIPG cells in vitro.

Conclusion·Novel targeted therapeutic strategies for DIPG has been identified through single drug and combination drug screening, providing basis for furthervalidation in vivo and therapeutic mechanism exploration.

Key words: diffuse intrinsic pontine glioma (DIPG), single targeted small molecule compounds screening, YM155, baculoviral IAP repeat containing 5 (BIRC5), drug combinatory screening

中图分类号:

R739.41

李瑞, 韩雨洁, 张蕾, 唐玉杰. 弥漫内生性脑桥胶质瘤靶向治疗新策略的体外筛选与验证[J]. 上海交通大学学报(医学版), 2021, 41(8): 987-998.

Rui LI, Yu-jie HAN, Lei ZHANG, Yu-jie TANG. Invitro screening and validation of novel targeted therapeutic strategy against diffuse intrinsic pontine glioma[J]. JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE), 2021, 41(8): 987-998.

图/表 5

图1 DIPG肿瘤组织和正常脑组织的转录组分析Note： A/B. Heatmap (A) and volcano plot (B) showing DEGs (|Log2FC|≥1, padj≤0.05) of DIPG versus normal cerebral cortex. C/D. GO (C) and Pathway (D) analysis identifying enriched function or pathway in up- or down-regulated genes of DIPG.

Fig 1 Transcriptome analyses of DIPG specimen and normal cerebral cortex

图2 SU_DIPG13细胞中小分子靶向药物的体外筛选Note： A. Classification of small molecule compounds used in drug screening. BETi—BET inhibitor; HDACi—HDAC inhibitor. B. Heatmap showing inhibition rates of cell viability by each compound at indicated concentrations in drug screening of SU_DIPG13 cells. Values up to standard (inhibition rate≥50% ) are shown in green. C. Ranks of compounds from drug screening based on inhibition rates at 1 μmol·L-1. The names of top 5 compounds are labeled. D. Heatmap showing expression levels in DIPG versus normal cerebral cortex of target genes of the top 11 compounds with inhibition rates more than 50% at 1 μmol·L-1. Values up to standards (log2FC≥1, padj≤0.05) are shown in green.HSP90AA1—heat shock protein 90 α family class A member 1; HSP90AB1—heat shock protein 90 α family class B member 1; PSMA—archaeal proteasome endopeptidase complex subunit α; PSMB—archaeal proteasome endopeptidase complex subunit β; PSMD—proteasome 26S subunit; FGFR1—fibroblast growth factor receptor 1; JARID1A—lysine demethylase 5A; JMJD3—lysine demethylase 6B; JMJD2A—lysine demethylase 4A; JMJD2B—lysine demethylase 4B.

Fig 2 Drug screening in SU_DIPG13 cells with selected targeted small molecule compounds in vitro

表1 在1 μmol·L-1条件下对SU_DIPG13细胞抑制率大于50%的药物

Tab 1 Drugs with growth inhibition rate over 50% at 1 μmol·L-1 in SU_DIPG13 cells

Drug	Inhibition rate/%	Target gene	IC₅₀/（μmol·L^-1）	Clinical trial	NCT number
YM155	98.97	BIRC5	0.004	Phase Ⅰ/Ⅱ	NCT01023386， NCT00818480
AUY922	85.01	HSP90AA1， HSP90AB1	0.050	Phase Ⅱ	NCT01854034
THZ1/CDK7 inhibitor	74.26	CDK7	0.280	Phase Ⅰ	NCT04247126
CDK1/2 inhibitor Ⅲ	73.27	CDK1， CDK2	0.400	Phase Ⅰ/Ⅱ	NCT02095132
Dinaciclib	72.99	CDK1， CDK2， CDK5， CDK9	0.010	Phase Ⅰ	NCT03484520
PR-171	70.69	PSMA， PSMB， PSMD	0.140	Phase Ⅰ/Ⅱ	NCT00150462， NCT00884312
Panobinostat	69.95	HDAC1， HDAC2， HDAC5， et al	0.240	Phase Ⅰ/Ⅱ/Ⅲ	NCT01496118， NCT04326764
Flavopiridol	66.49	CDK1， CDK2， CDK4， CDK6， CDK9	0.200	Phase Ⅰ/Ⅱ	NCT00445341
LY2874455	61.49	FGFR1， FGFR2， FGFR3， FGFR4	0.140	Phase Ⅰ	NCT03125239
INK 128	59.47	mTOR	0.170	Phase Ⅰ/Ⅱ	NCT02987959， NCT03097328
JIB-04	52.54	JARID1A， JMJD2E， JMJD3， JMJD2A， JMJD2B， et al	0.740	‒	‒

图3 靶向BIRC5的小分子药物YM155对DIPG的体外抑制作用Note： A. qRT-PCR analysis of BIRC5 in SU_DIPG13 cells treated with YM155 at indicated concentrations for 24 h. ①P=0.000. B. Western blotting detecting survivin expression in cell extracts from SU_DIPG13 cells treated with YM155 at indicated concentrations for 48 h. Tubulin is shown as loading control. C. Dosage curves of YM155 in treating SU_DIPG13 cells, SU_DIPG17 cells or mNSCs. IC50 of each line is shown. D. EdU staining detecting cell proliferation of SU_DIPG13 cells treated with YM155 at indicated concentrations for 24 h. E. Annexin V/PI staining detecting cell apoptosis of SU_DIPG13 cells treated with YM155 at indicated concentrations for 48 h.

Fig 3 Inhibitory effect of BIRC5-targeted inhibitor YM155 on DIPG in vitro

图4 DIPG的体外药物组合筛选及验证Note： A. Heatmap showing inhibition rates of combination screening at indicated concentrations. Inhibitory rates conforming to standard highlighted in green. B/C. Cell viability and synergy measurement in combinatory drug treatments. SU_DIPG13 (B) or SU_DIPG17 (C) cells were treated with panobinostat, ABT-737, JQ1 or CX4945 individually or in combination at indicated concentrations for 72 h.

Fig 4 Screening and validationof combinatory drug for DIPG in vitro

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