上海交通大学学报（医学版）

›› 2009, Vol. 29 ›› Issue (7): 813-.

• 论著（基础研究） • 上一篇    下一篇

磷酸氯喹诱导U937细胞凋亡机制的研究

刘 佳1, 陈芳源1, 王海嵘1, 钟济华1, 王利民2, 钟 华1, 韩洁英1, 欧阳仁荣1   

  1. 上海交通大学 医学院仁济医院 1.血液科上海血液学研究所, 2.中心实验室  上海 200001
  • 出版日期:2009-07-25 发布日期:2009-09-16
  • 通讯作者: 王海嵘, 电子信箱：whr75@sohu.com。
  • 作者简介:刘 佳（1980—）, 女, 住院医师, 硕士生；电子信箱: xiaxiaoyufar@hotmail.com。
  • 基金资助:

    国家自然科学基金（30670881）；仁济医院-交通大学基础医学院合作研究基金项目（ZD0704）

Research on mechanism of chloroquine phosphate-induced U937 cell apoptosis

LIU Jia1, CHEN Fang-yuan1, WANG Hai-rong1, ZHONG Ji-hua1, WANG Li-ming2, ZHONG Hua1, HAN Jie-ying1, OUYANG Ren-rong1   

  1. 1. Department of Hematology, Shanghai Institute of Hematology, 2. Center Laboratory, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200001, China
  • Online:2009-07-25 Published:2009-09-16
  • Supported by:

    National Natural Science Foundation of China, 30670881；Research Foundation of Renji Hospital-Shanghai Jiaotong University School of Medicine, ZD0704

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摘要：

目的 将磷酸氯喹作用于白血病细胞株U937，观察其对白血病细胞凋亡的影响；并研究磷酸氯喹是否通过逆转凋亡相关基因PNAS-2蛋白在白血病细胞中异常的亚细胞定位，从而促进细胞凋亡的机制。方法 将不同剂量的磷酸氯喹加入体外培养至对数生长期的白血病细胞株U937培养液中。MTT法检测细胞增殖；流式细胞仪和激光共聚焦显微镜检测细胞凋亡，同时观察磷酸氯喹对U937细胞中PNAS-2蛋白亚细胞定位的影响。结果 50 μg /mL磷酸氯喹可显著促进U937细胞发生凋亡，并使白血病细胞中PNAS-2蛋白的异常亚细胞定位发生改变。结论 磷酸氯喹对白血病细胞株U937有促进凋亡的作用，其凋亡机制可能是使PNAS-2蛋白在白血病细胞中异常的亚细胞定位恢复正常，对于临床治疗白血病的具有潜在的应用价值。

关键词: 磷酸氯喹, 凋亡, PNAS-2, 白血病, 亚细胞定位

Abstract:

Objective To observe the effects of chloroquine phosphate on apoptosis of leukemic cell line U937, and investigate whether chloroquine phosphate induces leukemic cell apoptosis by normalizing protein PNAS-2's abnormal subcellular location. Methods Chloroquine phosphate of different concentrations were added into culture fluid of leukemic cell line U937 at logarithmic phase. MTT was used to measure cell proliferation, flow cytometry and laser confocal microscopy were applied to detect cell apoptosis, and immunofluorescence technology was employed to observe the effects of chloroquine phosphate on the changes of subcellular location of protein PNAS-2. Results Apoptosis of leukemic cell line U937 was significantly induced by 50 μg/mL chloroquine phosphate, and subcellular location of protein PNAS-2 was changed. Conclusion Chloroquine phosphate can induce apoptosis of leukemic cell line U937, and the mechanism may be related to the normalization of PNAS-2's abnormal subcellular location in U937 cell line. Chloroquine phosphate has the potential to be used in leukemic therapy.

Key words: chloroquine phosphate, apoptosis, PNAS-2, leukemia, subcellular localization