上海交通大学学报（医学版）

›› 2009, Vol. 29 ›› Issue (8): 909-.

• 论著（基础研究） • 上一篇    下一篇

低氧对小鼠成骨细胞Cyr61/CCN1表达的影响

崔 论, 王 君, 齐 进, 朱雅萍, 许福平, 魏 立, 张凤华, 邓廉夫   

  1. 上海交通大学 医学院瑞金医院骨科 上海市伤骨科研究所, 上海 200025
  • 出版日期:2009-08-25 发布日期:2009-09-27
  • 作者简介:崔论(1984—), 男, 硕士生；电子信箱: clbelieve@yahoo.com.cn。
  • 基金资助:

    国家自然科学基金（30672145）和上海市创新计划（07dz22008）

Effects of hypoxia on expression of Cyr61/CCN1 in murine osteoblasts

CUI Lun, WANG Jun, QI Jin, ZHU Ya-ping, XU Fu-ping, WEI Li, ZHANG Feng-hua, DENG Lian-fu   

  1. Shanghai Institute of Traumatology and Orthopaedics, Department of Orthopedics, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200025, China
  • Online:2009-08-25 Published:2009-09-27
  • Supported by:

    National Natural Science Foundation of China, 30672145; Shanghai Innovation Project, 07dz22008

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摘要：

目的 探讨低氧环境对小鼠成骨细胞富含半胱氨酸61（Cyr61/CCN1）表达的影响。 方法 分别在氧浓度和去铁敏诱导的低氧环境下培养小鼠成骨细胞，Real-time PCR和Western blotting于不同时间点检测成骨细胞低氧诱导因子-1α（HIF-1α）、Cyr61/CCN1和血管内皮生长因子的表达。分别敲除小鼠成骨细胞中HIF-1α和VHL基因，检测细胞Cyr61/CCN1的表达。 结果 两种低氧环境下，小鼠成骨细胞HIF-1α通路被激活，Cyr61/CCN1 mRNA和蛋白表达均显著增加（P<0.05或P<0.01）。HIF-1α基因敲除小鼠成骨细胞Cyr61/CCN1 mRNA和蛋白表达均显著减少，VHL基因敲除成骨细胞Cyr61/CCN1 mRNA和蛋白表达均显著增加（P<0.05或P<0.01）。 结论 低氧环境通过激活HIF-1α通路来上调小鼠成骨细胞Cyr61/CCN1的表达。

关键词: 低氧, 低氧诱导因子- 1α, 成骨细胞, Cyr61/CCN1, 血管内皮生长因子

Abstract:

Objective To investigate the effects of hypoxia on the expression of cysteine-rich protein 61(Cyr61/CCN1) in murine osteoblasts. Methods Murine osteoblasts were cultured under oxygen of lower concentration or treated with desferrioxamine, and Real-time PCR and Western blotting were employed to detect the expression of HIF-1α, Cyr61/CCN1 and vascular endothelial growth factor in osteoblasts at different time points. HIF-1α gene or VHL gene were knocked out in murine osteoblasts, and the expression of Cyr61/CCN1 in murine osteoblasts was detected. Results HIF-1α pathway was activated in murine osteoblasts under the condition of hypoxia, and the expression of Cyr61/CCN1 in osteoblasts was significantly increased （P<0.05 or P<0.01）. The expression of Cyr61/CCN1 was significantly decreased in murine osteoblasts with HIF-1α gene knockout, and that was significantly increased in osteoblasts with VHL gene knockout （P<0.05 or P<0.01）. Conclusion The expression of Cyr61/CCN1 in murine osteoblasts may be up-regulated by hypoxia through activation of HIF-1α pathway.

Key words: hypoxia, hypoxia inducible factor-1α, osteoblasts, Cyr61/CCN1, vascular endothelial growth factor

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