上海交通大学学报（医学版）

›› 2009, Vol. 29 ›› Issue (11): 1320-.

• 论著（基础研究） • 上一篇    下一篇

DHPG诱导的大鼠海马脑片癫癎样放电模型基因表达谱分析

陆钦池1,2, 荆 里2, 陈生弟1   

  1. 上海交通大学 医学院 1. 瑞金医院神经内科, 上海 200025；2. 仁济医院神经内科, 上海 200127
  • 出版日期:2009-11-25 发布日期:2009-11-24
  • 通讯作者: 陈生弟, 电子信箱: chen_sd@medmail.com.cn。
  • 作者简介:陆钦池(1963—), 男, 主任医师, 博士生；电子信箱: qinchilu2005@yahoo.com.cn。
  • 基金资助:

    上海交通大学医工交叉基金（YG2007MS07）；上海市卫生局课题（054039）

Microarray analysis of DHPG-induced rat hippocampal slice epileptic seizure model

LU Qin-chi1,2, JING Li2, CHEN Sheng-di1   

  1. 1. Department of Neurology, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200025, China|2. Department of Neurology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200127, China
  • Online:2009-11-25 Published:2009-11-24
  • Supported by:

    Shanghai Jiaotong University Med-X Fund, YG2007MS07; Shanghai Municipal Health Bureau Foundation, 054039

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摘要：

目的 研究代谢型谷氨酸受体-Ⅰ（mGluR-Ⅰ）激动剂D-对羟基苯甘氨酸（DHPG）诱导的大鼠离体海马脑片癫癎样放电模型的基因表达谱。方法 以含50 μmol DHPG的人工脑脊液持续灌流大鼠离体海马脑片，诱导建立癫癎样放电模型（DHPG组,n=3）。采用cDNA微阵列分析技术获得DHPG组的基因表达谱，与对照组（n=3）比较筛选出差异表达基因，并进行富集功能分类。结果 与对照组比较，DHPG组样本模型分别筛选出的上调基因206个，下调基因489个；其中差异表达达1.5倍的上调基因67个，下调基因86个；2.0倍以上的上调基因6个，0.5倍以下的下调基因25个。富集功能分类结果显示，差异表达基因功能涉及蛋白结合（19个）、分子功能、钙离子结合和核苷酸结合等多方面。结论 癫癎样放电及mGluR-Ⅰ激动剂的作用均涉及多种基因，是一复杂的调控过程。实验为进一步研究提供了依据。

关键词: 代谢型谷氨酸受体, D-对羟基苯甘氨酸, 微阵列分析, 基因表达, 海马脑片, 癫癎

Abstract:

Objective To investigate the gene expression pattern of metabotropic glutamate receptor-Ⅰ(mGluR-Ⅰ), D-p-hydroxyphenylglycine (DHPG)-induced rat hippocampal slice epileptic seizure model. Methods In vitro rat hippocampal sclice was continously perfused with artificial cerebrospinal fluid containing 50 μmol DHPG, and epileptic seizure model was established (DHPG group, n=3). cDNA microarray chip was applied to explore the gene expression pattern in DHPG group, the differentially expressed genes were screened in comparison with control group (n=3), and functional classification analysis was conducted. Results There were 206 up-regulated genes and 489 down-regulated genes, among which 67 up-regulated genes and 86 down-regulated genes differentially expressed by 1.5 fold, 6 up-regulated genes differentially expressed by more than 2.0 folds, and 25 down-regulated genes differentially expressed by less than 0.5 fold. Functional classification analysis revealed that differentially expressed gene function involved in protein binding (19 genes), molecular function, calciumion binding and nucleotide binding. Conclusion Epileptic seizure and roles of mGluR-Ⅰagonist may be related to various genes, which is a complicated process. This experiment provides evidences for further researches.

Key words: metabotropic glutamate receptor, D-p-hydroxyphenylglycine, microarray, gene expression, hippocampal sclice, epilepsy