上海交通大学学报（医学版）

›› 2009, Vol. 29 ›› Issue (12): 1410-.

• 论著（基础研究） • 上一篇    下一篇

二水磷酸氢钙涂层镁锌合金体内降解和新骨形成的研究

陶海荣1, 顾剑华1, 何耀华2, 张 岩2, 张绍翔3, 宋 阳3, 李佳楠3, 赵常利2, 蒋 垚2, 张小农3   

  1. 上海交通大学 1. 医学院第三人民医院骨科, 上海 201900；2. 第六人民医院骨科, 上海 200233；3. 材料科学与工程学院, 上海 200240
  • 出版日期:2009-12-25 发布日期:2009-12-25
  • 通讯作者: 蒋 垚, 电子信箱: ortho.jiangyao@163.com。
  • 作者简介:陶海荣（1975—）, 男, 副主任医师, 博士；电子信箱: taohair@163.com。
  • 基金资助:

    国家自然科学基金（30772182）和上海市卫生局基金(2007Y24)

Degradation of dicalcium phosphate dihydrate-coated Mg-Zn alloy and bone formation in vivo

TAO Hai-rong1, GU Jian-hua1, HE Yao-hua2, ZHANG Yan2, ZHANG Shao-xiang3, SONG Yang3, LI Jia-nan3, ZHAO Chang-li2, JIANG Yao2, ZHANG Xiao-nong3   

  1. 1. Department of Orthopedics, The Third People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 201900, China；2. Department of Orthopedics, The Sixth People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200233, China；3. Materials Science and Engineering, Shanghai Jiaotong University, Shanghai 200240, China
  • Online:2009-12-25 Published:2009-12-25
  • Supported by:

    National Natural Science Foundation of China, 30772182; Shanghai Municipal Health Bureau Foundation, 2007Y24

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摘要：

目的 研究二水磷酸氢钙涂层镁锌合金在体内的降解规律及其对新骨形成的影响。方法 72只新西兰大白兔均于左侧股骨髁钻孔，随机植入二水磷酸氢钙涂层镁锌合金棒(实验组，n=24)或镁锌合金棒(镁锌合金对照组，n=24)或聚乳酸棒(聚乳酸对照组，n=24)。术前1 d及术后第1天和第1、2、5、10周时，检测实验组和镁锌合金对照组动物血清Mg2+浓度。术后第3、6、12、18周时，植入处X线摄片检查；摄片后三组分别处死6只动物，采集并制备肝、肾组织标本，HE染色后观察组织病理学改变。术后18周时，行股骨髁组织切片HE染色和品红苦味酸染色，观察植入物降解情况并比较骨矿物质沉积率。结果 二水磷酸氢钙涂层组与镁锌合金对照组术前及术后各时间点的血清Mg2+浓度比较，差异均无统计学意义（P>0.05）。术后3周时，X线摄片观察发现镁锌合金对照组植入物旁有气泡产生。三组肝、肾组织学检查均未见明显异常。与两对照组相比，实验组骨矿物质沉积率较高，植入物的降解速度较慢。结论 二水磷酸氢钙涂层镁锌合金体内降解时间延长，生物相容性更优良。

关键词: 二水磷酸氢钙, 涂层, 镁锌合金, 降解, 骨形成

Abstract:

Objective To investigate the degradation of dicalcium phosphate dehydrate-coated Mg-Zn alloy in vivo and bone formation. Methods Left femoral condyles were drilled in 72 New Zealand rabbits, and were randomly divided into experiment group (n＝24, implanted with dicalcium phosphate dehydrate-coated Mg-Zn alloy rods), Mg-Zn alloy control group (n＝24, implanted with Mg-Zn alloy rods) and poly-L-lactide acid rod group (n＝24, implanted with poly-L-lactide acid rods). Serum concentrations of Mg2+ were examined 1 d pre-operation, and 1 d, 1 week, 2 weeks, 5 weeks and 10 weeks post-operation in experiment group and Mg-Zn alloy control group. Operation sites were examined by X-rays at 3, 6, 12 and 18 weeks post-operation. After X-ray examination at each time point, 6 rabbits in each group were sacrificed, and subjected to histopathological observation of live and kidney tissues by HE staining. Tissues from condyles of femur were observed by HE staining and 2, 4, 6-trinitrophenol rosein staining, and mineral apposition rate of bone was calculated. Results There was no significant difference in the concentrations of serum Mg2+ at each time point between Mg-Zn alloy control group and experiment group (P>0.05). X-ray examination revealed gas emerged near the implants 3 weeks after surgery in Mg-Zn alloy control group. However, there was no obvious histological abnormality in liver and kidney tissues. The mineral apposition rate was higher and the degradation of material was lower in experiment group than those in the other two groups. Conclusion Dicalcium phosphate dehydrate-coated Mg-Zn alloy has a favourable biocompatibility, and degrades more slowly in vivo.

Key words: hydryoxyapetite, coat, Mg-Zn alloy, degradation, bone formation