上海交通大学学报（医学版）

›› 2010, Vol. 30 ›› Issue (2): 158-.

• 专题报道（妇科疾病研究） • 上一篇    下一篇

拓扑替康、顺铂和泰素对宫颈癌HeLa细胞的抑制及放射增敏作用

张文璎, 薛月珍, 陶敏芳   

  1. 上海交通大学 第六人民医院妇产科, 上海 200233
  • 出版日期:2010-02-25 发布日期:2010-02-25
  • 通讯作者: 薛月珍, 电子信箱: yuezhenxue@hotmail.com。
  • 作者简介:张文璎（1981—）, 男, 硕士；电子信箱: zhang_wen_y@126.com；现在上海市长宁区妇幼保健院(200051)。

Inhibition and radiosensitization of Topotecan, Cisplatin and Taxol on cervical cancer cell line HeLa

ZHANG Wen-ying, XUE Yue-zhen, TAO Min-fang   

  1. Department of Obstetrics and Gynecology, The Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 200233, China
  • Online:2010-02-25 Published:2010-02-25

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摘要：

目的 研究拓扑替康（TPT）对宫颈癌HeLa细胞的杀伤作用和放射增敏作用，并与顺铂（DDP）和泰素（TAX）进行对比分析。方法 采用MTT法检测TPT、DDP和TAX对宫颈癌HeLa细胞增殖能力的影响，细胞克隆形成法研究三种化疗药物的放射增敏作用，并应用单击多靶模型计算放射增敏比。结果 TPT、DDP和TAX作用HeLa细胞24、48和72 h半数抑制浓度分别为8.0、2.6和0.8 μg/mL，2.4、0.7和0.1μg/mL，0.3、0.1和0.0 μg/mL。三种化疗药物的放射增敏组肿瘤细胞凋亡率均明显高于单放射组和单独化疗组（P<0.05）；放射增敏实验中，TPT作用24 h和48 h后放射增敏比分别为1.167和1.344，DDP为1.314和1.538，TAX为1.076和1.316。结论 TPT、DDP和TAX对宫颈癌HeLa细胞具有明显抑制作用，且呈时间和浓度依赖性。三种化疗药物具有放射增敏作用，DDP的放射增敏作用最强，TAX最弱。

关键词: 拓扑替康, 顺铂, 泰素, HeLa细胞, 放射增敏

Abstract:

Objective To investigate the inhibition and radiosensitization effects of Topotecan (TPT) on cervical cancer cell line HeLa, and make comparison with Cisplatin (DDP) and Taxol (TAX). Methods The effects of TPT, DDP and TAX on proliferation of cervical cancer cell line HeLa were evaluated by MTT assay. The radiosensitization effects of TPT, DDP and TAX on HeLa cells were detected by clone formation assay, and the sensitization enhancement ratios of TPT, DDP and TAX were calculated. Results The IC50 of TPT at different reaction time (24 h, 48 h and 72 h) were 8.0 μg/mL, 2.6 μg/mL and 0.8 μg/mL, respectively, those of DDP were 2.4 μg/mL, 0.7 μg/mL and 0.1 μg/mL, respectively, and those of TAX were 0.3 μg/mL, 0.1 μg/mL and 0.0 μg/mL, respectively. The apoptosis rates of tumor cells treated by radiosensitization were significantly higher than those of tumor cells treated by single radiotherapy or single chemotherapy (P<0.05). The sensitization enhancement ratios of TPT at 24 h and 48 h were 1.167 and 1.344, respectively, those of DDP were 1.314 and 1.538, respectively, and those of TAX were 1.076 and 1.316, respectively. Conclusion TPT, DDP and TAX have significant inhibition effects on cervical cancer cell line HeLa, which are time-dependant and dose-dependant. Besides, TPT, DDP and TAX have radiosensitizing effects, with DDP being the strongest and TAX being the weakest.

Key words: Topotecan, Cisplatin, Taxol, HeLa cell line, radiosensitization