上海交通大学学报（医学版）

›› 2010, Vol. 30 ›› Issue (2): 186-.

• 论著（基础研究） • 上一篇    下一篇

益生菌对炎症性肠病小鼠肠道菌群紊乱及细菌移位的影响

王坚镪1, 丁在咸2, 张 旻3, 陈红旗4, 秦环龙4, 蒋燕群1   

  1. 上海交通大学 第六人民医院 1. 检验科, 2. 动物实验中心, 上海 200233；3. 上海交大昂立医药研究院, 上海 200233；4. 上海交通大学 第六人民医院普外科, 上海 200233
  • 出版日期:2010-02-25 发布日期:2010-02-25
  • 通讯作者: 陈红旗, 电子信箱: hqchen08@hotmail.com。
  • 作者简介:王坚镪（1972—）, 男, 主管技师, 学士；电子信箱: jqwang01@163.com。丁在咸（1954—）, 男, 主管技师；电子信箱: dingzaixian@sina.com。
  • 基金资助:

    黎介寿院士肠屏障基金（LJS_2009001）

Effect of probiotics on intestinal flora disturbance and bacterial translocation in mice with spontaneous colitis

WANG Jian-qiang1, DING Zai-xian2, ZHANG Min3, CHEN Hong-qi4, QIN Huan-long4, |JIANG Yan-qun1   

  1. 1. Department of Clinical Laboratory, 2. Department of Experimental Animals, The Sixth People's Hospital, 3. Institute of Bio-Medicine, 4. Department of General Surgery, The Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 200233, China
  • Online:2010-02-25 Published:2010-02-25
  • Supported by:

    LI Jie-shou academician Foundation, LJS_2009001

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摘要：

目的 探讨植物乳杆菌（LP）对炎症性肠病（IBD）小鼠肠道菌群及细菌移位的影响。方法 采用白介素10基因敲除(IL-10-/-)小鼠作为IBD动物模型，将8周龄雌性小鼠随机分成空白对照组、IL-10-/-组和IL-10-/-+LP组三组。IL-10-/-+LP组每日灌胃0.5 mL LP菌液（1.0×109 CFU/mL），其余两组灌胃Ringer缓冲液0.5 mL，持续4周。以小鼠粪便中的双歧杆菌、乳酸杆菌、肠杆菌和产气荚膜梭菌数量及肠系膜淋巴结、脾脏细菌移位为检测指标。结果 IL-10-/-小鼠肠内双歧杆菌、乳酸杆菌含量明显下降，肠球菌、产气荚膜梭菌含量升高，且肠道细菌移位明显增加；而连续灌胃LP菌液4周后，益生菌发挥了对肠道的调节作用，纠正了肠道菌群失衡，并降低了肠道细菌移位。结论 LP能纠正炎症性肠病小鼠肠道菌群紊乱，减少细菌移位，从而增强了肠道屏障功能。

关键词: 益生菌, 植物乳杆菌CGMCC 1258, 菌群紊乱, 细菌移位

Abstract:

Objective To investigate the effect of Lactobacillus plantarum (LP) on intestinal flora and bacterial translocation in mice with spontaneous inflammatory bowel disease (IBD). Methods Interleukin 10 knockout mice (IL-10-/-) were used as models of IBD. Eight-week old female mice were randomized to control group, IL-10-/- group and IL-10-/-+LP group. IL-10-/-+LP group received 0.5 mL LP (1.0×109 CFU/mL) per day for 4 weeks, and the other groups received 0.5 mL Ringer buffer. Intestinal flora including Bifidobacteria, Lactobacilli, Enterobacteriaceae and Clostridium perfringens in the feces and bacterial translocation in mesenteric lymph nodes and spleens were detected. Results The contents of Bifidobacteria and Lactobacilli significantly decreased in the intestine of IL-10-/- mice, while those of Enterobacteriaceae and Clostridium perfringens significantly increased, and the bacterial translocation significantly increased. Four weeks after LP treatment, the disturbed intestinal flora was restored, and the bacterial translocation decreased. Conclusion LP administration can modulate the imbalance of intestinal flora and decrease the bacterial translocation, thus enhance intestinal barrier function in mice with IBD.

Key words: probiotics, Lactobacillus plantarum CGMCC 1258, intestinal flora disturbance, bacterial translocation