上海交通大学学报（医学版）

›› 2011, Vol. 31 ›› Issue (1): 5-.doi: 10.3969/j.issn.1674-8115.2011.01.002

• 论著（基础研究） • 上一篇    下一篇

口腔鳞状细胞癌细胞TLR3表达及其配体poly(I:C）的诱导凋亡作用

罗清琼1, 陈万涛2, 胡水清1, 陈福祥1   

  1. 上海交通大学 医学院附属第九人民医院 1.检验科, 2.上海市口腔医学重点实验室, 上海 200011
  • 出版日期:2011-01-28 发布日期:2011-02-01
  • 通讯作者: 陈福祥, 电子信箱: fuxiang_chen@hotmail.com。
  • 作者简介:罗清琼（1985—）, 女, 硕士；电子信箱: luoqingqiong2007@126.com。
  • 基金资助:

    上海市浦江人才计划（09PJ1407100）；教育部留学回国人员科研启动基金（20091001）；上海市自然科学基金（08ZR1412700）；上海市重点学科开放基金(S30206)

Expression of TLR3 in oral squamous carcinoma cells and induction of apoptosis by its ligand poly(I:C）

LUO Qing-qiong1, CHEN Wan-tao2, HU Shui-qing1, CHEN Fu-xiang1   

  1. 1.Department of Laboratory, 2.Shanghai Key Laboratory of Stomatology, The Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, China
  • Online:2011-01-28 Published:2011-02-01
  • Supported by:

    Shanghai Pujiang Talent Program, 09PJ1407100；Foundation for Returned Overseas from The Ministry of Education, 20091001；Shanghai Natural Science Foundation, 08ZR1412700；Shanghai Key Discipline Foundation, S30206

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摘要：

目的 研究口腔鳞状细胞癌细胞中Toll样受体3（TLR3）的表达及其配体poly （I:C）的生物学作用。方法 RT-PCR和流式细胞术检测口腔鳞状细胞癌细胞系CAL-27和HB中TLR3 mRNA和蛋白表达。以100 μg/mL poly（I:C）处理CAL-27， 分别于刺激后24、48和72 h时点采用MTT比色法测定细胞活力，24 h时点采用流式细胞术检测细胞凋亡；并设立空白对照。结果 CAL-27和HB中TLR3 mRNA和蛋白均呈高表达。CAL-27经poly(I:C）处理后，各检测时点的细胞活力均显著低于空白对照细胞（P<0.05）；细胞凋亡率明显高于空白对照细胞[(16.67±1.202）% vs (7.00±1.155)%]（P<0.05）。结论 口腔鳞状细胞癌细胞内高表达TLR3，其配体poly(I:C）能诱导口腔鳞状细胞癌细胞凋亡。

关键词: poly （I:C）, Toll样受体3, 口腔鳞状细胞癌, 细胞凋亡

Abstract:

Objective To investigate the expression of Toll-like receptor 3 (TLR3) and the biological characteristics of its ligand poly (I:C) in oral squamous carcinoma cells. Methods The expression of TLR3 mRNA and protein in oral squamous carcinoma CAL-27 cells and HB cells was detected by RT-PCR and flow cytometry. CAL-27 cells were treated with 100 μg/mL poly (I:C), cell viability was determined by MTT assay at 24, 48 and 72 h after stimulation, and cell apoptosis was detected by flow cytometry. Besides, blank controls were established. Results TLR3 mRNA and protein were highly expressed in CAL-27 and HB cells. After treatment by poly (I:C), the viability of CAL-27 cells was significantly lower than that of blank controls at each time point (P<0.05), and the apoptosis rate of CAL-27 cells was significantly higher than that of blank controls [(16.67±1.202）% vs (7.00±1.155)%]（P<0.05）. Conclusion TLR3 is highly expressed in oral squamous carcinoma cells, and its ligand poly(I:C) can induce the apoptosis of oral squamous carcinoma cells.

Key words: poly (I:C), Toll-like receptor, oral squamous carcinoma, apoptosis