›› 2011, Vol. 31 ›› Issue (1): 5-.doi: 10.3969/j.issn.1674-8115.2011.01.002

• 论著(基础研究) • 上一篇    下一篇


罗清琼1, 陈万涛2, 胡水清1, 陈福祥1   

  1. 上海交通大学 医学院附属第九人民医院 1.检验科, 2.上海市口腔医学重点实验室, 上海 200011
  • 出版日期:2011-01-28 发布日期:2011-02-01
  • 通讯作者: 陈福祥, 电子信箱: fuxiang_chen@hotmail.com。
  • 作者简介:罗清琼(1985—), 女, 硕士;电子信箱: luoqingqiong2007@126.com。
  • 基金资助:


Expression of TLR3 in oral squamous carcinoma cells and induction of apoptosis by its ligand poly(I:C)

LUO Qing-qiong1, CHEN Wan-tao2, HU Shui-qing1, CHEN Fu-xiang1   

  1. 1.Department of Laboratory, 2.Shanghai Key Laboratory of Stomatology, The Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, China
  • Online:2011-01-28 Published:2011-02-01
  • Supported by:

    Shanghai Pujiang Talent Program, 09PJ1407100;Foundation for Returned Overseas from The Ministry of Education, 20091001;Shanghai Natural Science Foundation, 08ZR1412700;Shanghai Key Discipline Foundation, S30206


目的 研究口腔鳞状细胞癌细胞中Toll样受体3(TLR3)的表达及其配体poly (I:C)的生物学作用。方法 RT-PCR和流式细胞术检测口腔鳞状细胞癌细胞系CAL-27和HB中TLR3 mRNA和蛋白表达。以100 μg/mL poly(I:C)处理CAL-27, 分别于刺激后24、48和72 h时点采用MTT比色法测定细胞活力,24 h时点采用流式细胞术检测细胞凋亡;并设立空白对照。结果 CAL-27和HB中TLR3 mRNA和蛋白均呈高表达。CAL-27经poly(I:C)处理后,各检测时点的细胞活力均显著低于空白对照细胞(P<0.05);细胞凋亡率明显高于空白对照细胞[(16.67±1.202)% vs (7.00±1.155)%](P<0.05)。结论 口腔鳞状细胞癌细胞内高表达TLR3,其配体poly(I:C)能诱导口腔鳞状细胞癌细胞凋亡。

关键词: poly (I:C), Toll样受体3, 口腔鳞状细胞癌, 细胞凋亡


Objective To investigate the expression of Toll-like receptor 3 (TLR3) and the biological characteristics of its ligand poly (I:C) in oral squamous carcinoma cells. Methods The expression of TLR3 mRNA and protein in oral squamous carcinoma CAL-27 cells and HB cells was detected by RT-PCR and flow cytometry. CAL-27 cells were treated with 100 μg/mL poly (I:C), cell viability was determined by MTT assay at 24, 48 and 72 h after stimulation, and cell apoptosis was detected by flow cytometry. Besides, blank controls were established. Results TLR3 mRNA and protein were highly expressed in CAL-27 and HB cells. After treatment by poly (I:C), the viability of CAL-27 cells was significantly lower than that of blank controls at each time point (P<0.05), and the apoptosis rate of CAL-27 cells was significantly higher than that of blank controls [(16.67±1.202)% vs (7.00±1.155)%](P<0.05). Conclusion TLR3 is highly expressed in oral squamous carcinoma cells, and its ligand poly(I:C) can induce the apoptosis of oral squamous carcinoma cells.

Key words: poly (I:C), Toll-like receptor, oral squamous carcinoma, apoptosis