上海交通大学学报（医学版）

›› 2011, Vol. 31 ›› Issue (5): 556-.doi: 10.3969/j.issn.1674-8115.2011.05.007

• 论著（基础研究） • 上一篇    下一篇

断骨膏外用对去势大鼠骨折后骨痂形成和改建的影响

许 勇, 李飞跃, 王晋申, 齐 进, 周 琦, 李中伟   

  1. 上海交通大学 医学院附属瑞金医院伤科 |上海市伤骨科研究所, 上海 200025
  • 出版日期:2011-05-28 发布日期:2011-05-27
  • 通讯作者: 李中伟, 电子信箱: lzw10474@rjh.com.cn。
  • 作者简介:许 勇(1975—), 男, 主治医师, 博士；电子信箱: xy11355@rjh.com.cn。
  • 基金资助:

    上海市科委自然基金（08ZR1417500）；国家自然科学基金青年项目（30801476）

Effects of topical application of Duan Gu Gao on bony callus development and reconstruction after fracture in ovariectomized rats

XU Yong, LI Fei-yue, WANG Jin-sheng, QI Jin, ZHOU Qi, LI Zhong-wei   

  1. Department of Traumatology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Institute for Traumatology and Orthopedics, Shanghai 200025, China
  • Online:2011-05-28 Published:2011-05-27
  • Supported by:

    Shanghai Science and Technology Committee Foundation, 08ZR141750；National Natural Science Foundation of China, 30801476

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摘要：

目的 观察外用断骨膏对骨质疏松性骨折早期骨痂形成的影响。方法 采用切除卵巢法制作大鼠骨质疏松模型（切除卵巢组，n=45），正常对照组（n=25）大鼠采用假手术。术后第3个月，两组各取5只大鼠验证造模情况；其余60只大鼠制作胫骨骨折模型，其中切除卵巢组分为骨质疏松对照组（外用空白对照药膏，n=20）和骨质疏松断骨膏组（外用三七断骨膏，n=20）。分别在骨折早期的第1、2、3、4周采用X线、组织形态学以及Micro-CT检测骨痂形成的情况。结果 切除卵巢术后第3个月，Micro-CT显示切除卵巢组大鼠骨小梁数目减少，间距增加，骨密度降低，说明骨质疏松造模成功。骨折后第2~4周时，骨质疏松对照组骨痂体积较大，但骨痂密度在第3周后明显低于正常对照组（P<0.05）；而断骨膏外用后，骨痂体积接近正常对照组，但在第3周后骨痂密度则明显高于骨质疏松对照组（P<0.05）。组织学观察显示，切除卵巢组软骨骨痂向骨性骨痂转化进程延缓，而外用断骨膏可以改善此过程。结论 在骨质疏松性骨折早期，断骨膏能够促进软骨骨痂向骨性骨痂转化形成，减少早期软骨骨痂的过量形成，提高骨痂的密度。

关键词: 断骨膏, 骨质疏松性骨折, 骨痂

Abstract:

Objective To investigate the effects of topical application of Duan Gu Gao on bony callus development in early stage after osteoporotic fracture. Methods Rat osteoporosis models were prepared by ovariectomy (ovariectomy group, n=45), and sham operations were performed in another 25 rats (normal control group).  Three months after operation, 5 rats were selected from each group, and model establishment was verified. The models of tibia fracture were constructed in the other 60 rats, and those in ovariectomy group were subdivided into osteoporosis control group (topical application of blank control ointment, n=20) and osteoporosis Duan Gu Gao group (topical application of San Qi Duan Gu Gao, n=20). The development of bony callus was detected by X ray, histomorphology and Micro-CT 1 week, 2 weeks, 3 weeks and 4 weeks after fracture. Results Three months after ovariectomy, Micro-CT revealed that rats in ovariectomy group had less bone trabecula, decreased space between bone trabecula and reduced bone density, and osteoporosis models were verified. Two to four weeks after fracture, the volume of bony callus in osteoporosis control group was larger, while the bone density 3 weeks after fracture was significantly lower that that in normal control group (P<0.05). After topical application of Duan Gu Gao, the volume of bony callus was similar to that in normal control group, and the bone density 3 weeks after fracture was significantly higher than that in osteoporosis control group (P<0.05). Histological observations indicated that the process from chondral callus to osseous callus delayed in ovariectomy group, which was improved by topical application of Duan Gu Gao. Conclusion Duan Gu Gao can improve the process from chondral callus to osseous callus in early stage after osteoporotic fracture, reduce the overdevelopment of chondral callus in early stage, and increase density of bone callus.

Key words: Duan Gu Gao, osteoporotic fracture, bony callus