上海交通大学学报（医学版）

›› 2011, Vol. 31 ›› Issue (5): 571-.doi: 10.3969/j.issn.1674-8115.2011.05.010

• 论著（基础研究） • 上一篇    下一篇

育肾养血方治疗卵巢早衰的实验研究

徐海霞, 胡国华, 夏亦冬   

  1. 上海市中医医院妇科, 上海 200071
  • 出版日期:2011-05-28 发布日期:2011-05-27
  • 通讯作者: 夏亦冬, 电子信箱: xyd55568@sina.com。
  • 作者简介:徐海霞（1982—）, 女, 硕士生；电子信箱: sally19821003@yahoo.com.cn。
  • 基金资助:

    上海中医药大学校级课题(2009047)；上海市卫生局中医药科研基金(2010J004A)

Experimental study of treatment of premature ovarian failure with YuShenYangXue decoction

XU Hai-xia, HU Guo-hua, XIA Yi-dong   

  1. Department of Gynecology, Shanghai Traditional Chinese Medicine Hospital, Shanghai 200071, China
  • Online:2011-05-28 Published:2011-05-27
  • Supported by:

    Shanghai University of Traditional Chinese Medicine Foundation, 2009047；Shanghai Municipal Health Bureau Foundation, 2010J004A

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摘要：

目的 探讨全国名老中医朱南孙教授的经验方——育肾养血方治疗卵巢早衰的疗效及作用机制。方法 采用75只性周期规律的雌性Wistar大鼠的卵巢组织制备卵巢抗原;另取50只大鼠随机分成正常组（n=10）和模型组（n=40）。模型组采用卵巢抗原皮下注射自身免疫法建立卵巢早衰大鼠模型,造模成功后再分为四组（n=10）：中药中、高剂量组（灌服中药复方）、西药对照组（灌服己烯雌酚）、模型对照组（灌服等量生理盐水）；正常组为空白对照组（不造模仅灌服等量生理盐水），均连续2周。治疗结束后经腹主动脉采血，留取卵巢组织标本。采用ELISA法检测血清雌二醇（E2）和促卵泡激素（FSH）水平，免疫组织化学法检测卵巢抑制素B（InhB）蛋白阳性表达水平，观察卵巢、子宫湿重变化及组织学改变。结果 育肾养血方能提高卵巢早衰大鼠血清E2水平和降低FSH水平;但与模型对照组比较，西药对照组的E2和FSH水平、中药高剂量组的FSH水平差异均有统计学意义（P<0.05）。与模型对照组比较,西药对照组和中药中、高剂量组大鼠卵巢InhB蛋白表达均显著增加（P<0.01）；卵巢内淋巴细胞浸润减轻，各级卵泡数增多，卵巢和子宫的湿重增加。结论 育肾养血方可以促进卵巢早衰大鼠的卵泡生长，增加InhB和E2的分泌，恢复受损卵巢的内分泌功能。

关键词: 育肾养血方, 卵巢早衰, 促卵泡激素, 雌二醇, 抑制素B

Abstract:

Objective To explore the outcomes and mechanisms of YuShenYangXue decoction designed by Professor ZHU Nansun in the treatment of premature ovarian failure. Methods Ovarian antigen was prepared by ovarian tissues of 75 female Wistar rats with regular sexual cycle. Another 50 female Wistar rats were randomly divided into normal group (n=10) and model group (n=40). Rat models of premature ovarian failure were established by autogenous immunization in model group, and were subdivided into 4 groups (n=10): middle-dose traditional Chinese medicine group and high-dose traditional Chinese medicine group (administered with middle-dose and high-dose traditional Chinese medicine compound for 2 weeks respectively), western medicine control group (treated with diethylstilbestrol for 2 weeks) and model control group (managed with same amount of normal saline for 2 weeks). Besides, rats in normal group were served as blank control group, and were managed with same amount of normal saline for 2 weeks. Blood samples were obtained from abdominal aorta after treatment, and samples of ovarian tissues were harvested. Serum levels of estradiol (E2) and follicle-stimulating hormone (FSH) were measured by ELISA, the expression of ovarian inhibin B（InhB）protein was detected by immunohistochemistry, and the changes of wet weight and histology of ovary and uterus were observed. Results YuShenYangXue decoction increased serum E2 level and decreased serum FSH level in rats with premature ovarian failure. There were significant differences in serum E2 level between model control group and western medicine group (P<0.05), and serum FSH level in model control group was significantly different from those in western medicine group and high-dose traditional Chinese medicine group (P<0.05). Compared with model control group, the expression of ovarian InhB protein significantly increased (P<0.01), the lymphocyte infiltration in ovary decreased, the number of growing follicles increased, and the wet weight of uterine and ovary increased in western medicine group and middle-dose and high-dose traditional Chinese medicine groups. Conclusion YuShenYangXue decoction can promote follicle growth in rats with premature ovarian failure, increase secretion of ovarian E2 and InhB, and improve endocrine function of damaged ovaries.

Key words: YuShenYangXue decoction, premature ovarian failure, follicle-stimulating hormone, estradiol, inhibin B