上海交通大学学报（医学版）

›› 2011, Vol. 31 ›› Issue (7): 890-.doi: 10.3969/j.issn.1674-8115.2011.07.005

• 论著（基础研究） • 上一篇    下一篇

卵巢切除小鼠骨折早期愈合延迟机制的初步探讨

赵 建1, 赵 强1, 张连方1, 邓若晛2, 齐 进1, 周 琦1, 王晋申1, 林柳兰3, 邓廉夫1   

  1. 1.上海交通大学 医学院附属瑞金医院上海市伤骨科研究所 上海市中西医结合防治骨与关节病损重点实验室, 上海 200025； 2.上海理工大学 医疗器械与食品学院, 上海 200093； 3.上海大学快速制造中心, 上海 200444
  • 出版日期:2011-07-28 发布日期:2011-07-27
  • 通讯作者: 邓廉夫, 电子信箱: lianfudeng@126.com。
  • 作者简介:赵 建（1982—）, 男, 硕士生；电子信箱: zhaojian426@163.com。
  • 基金资助:

    国家自然科学基金（30872641）和上海市研发基地项目（09dz2200500）

Preliminary study of mechanism of delayed early fracture healing in ovariectomized mice

ZHAO Jian1, ZHAO Qiang1, ZHANG Lian-fang1, DENG Ruo-xian2, QI Jin1, ZHOU Qi1, WANG Jin-shen1, LIN Liu-lan3, DENG Lian-fu1   

  1. 1.Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Institute of Traumatology and Orthopedics, Shanghai Key Laboratory of Combination of Traditional Chinese and Western Medicine in Prevention and Therapy of Osteo-arthropathy, Shanghai 200025, China；2.School of Medical Devices and Food Sciences, University of Shanghai for Science and Technology, Shanghai 200093, China；3.Rapid Manufacturing Center, Shanghai University, Shanghai 200444, China
  • Online:2011-07-28 Published:2011-07-27
  • Supported by:

    National Natural Science Foundation of China, 30872641；Shanghai Research and Development Base Project, 09dz2200500

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摘要：

目的 观察卵巢切除小鼠的骨折早期愈合情况，并探讨其可能的机制。方法 40只6周龄雌性C57BL/6小鼠随机分为卵巢切除组（OVX组）和假手术组，每组20只。于OVX组小鼠卵巢切除术后4周，两组分别建立右侧股骨骨折模型。建模后2周，X线摄片观察骨折愈合情况；Micro-CT测定骨痂体积和骨密度，观察血管灌注后骨折部位血管化情况并测定血管体积和血管体积分数；制作骨组织标本，HE染色光学显微镜观察骨折愈合进程，免疫组织化学染色观察骨痂组织血管内皮生长因子(VEGF)蛋白表达。结果 X线片观察和骨折标本组织学检查显示：OVX组小鼠早期骨折愈合明显较假手术组延迟。Micro-CT检测结果显示：与假手术组比较，OVX组骨痂体积较小，骨密度较低，血管灌注后骨折部位新生血管少且血管体积和血管体积分数较小，且差异均有统计学意义（P<0.05)。免疫组织化学染色观察发现OVX组骨痂组织中VEGF表达明显少于假手术组。结论 卵巢切除后股骨骨折小鼠的骨折早期愈合明显延迟，可能与骨痂组织新生血管减少有关。

关键词: 骨质疏松, 卵巢切除, 骨折愈合

Abstract:

Objective To observe the early fracture healing in ovariectomized mice, and explore the possible mechanism. Methods Forty female C57BL/6 mice aged 6 weeks were randomly divided into ovariectomy group (OVX group) and sham operation group, with 20 rats in each group. Four weeks after ovariectomy in OVX group, right femur fracture mouse models were established in both groups. Two weeks after model establishment, the fracture healing was observed by X-ray examinations, and Micro-CT was used to measure the volumes of callus and mineral density, observe the angiogenesis and determine the vessel volumes and vessel volume fractions in fracture sites after vessel perfusion. Bone tissue samples were prepared 2 weeks after model establishment, the fracture healing was observed by light microscopy with HE staining, and the expression of vascular endothelial growth factor (VEGF) protein in callus tissues was observed with immunohistochemical staining. Results X-ray examinations and histological examinations of fracture samples indicated that the early fracture healing in OVX group was significantly delayed compared with sham operation group. Micro-CT detection revealed that compared with sham operation group, the volume of callus, mineral density, and number of vessels, vessel volume and vessel volume fraction in fracture sites after vessel perfusion were significantly decreased (P<0.05). It was observed by immunohistochemical staining that the expression of VEGF in callus tissues in OVX group was significantly lower than that in sham operation group. Conclusion The early fracture healing is significantly delayed in ovariectomized mice, which may be related to the reduction of angiogenesis in callus tissues.

Key words: osteoporosis, ovariectomy, fracture healing