上海交通大学学报（医学版）

›› 2011, Vol. 31 ›› Issue (8): 1145-.doi: 10.3969/j.issn.1674-8115.2011.08.022

• 论著（临床研究） • 上一篇    下一篇

C型凝集素受体和Toll样受体在子前期重度患者胎盘中的表达

李 黎, 徐 亮, 滕银成   

  1. 上海交通大学附属第六人民医院妇产科, 上海 200233
  • 出版日期:2011-08-28 发布日期:2011-08-29
  • 通讯作者: 滕银成, 电子信箱: teng1008@sohu.com。
  • 作者简介:李 黎（1984—）, 女, 硕士生；电子信箱: obstetrics@163.com。
  • 基金资助:

    上海市科委基金（10411966500）

Expression of C-type lectin receptors and Toll-like receptors in placenta of severe pre-eclampsia

LI Li, XU Liang, TENG Yin-cheng   

  1. Department of Obstetrics and Gynecology, the Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 200233, China
  • Online:2011-08-28 Published:2011-08-29
  • Supported by:

    Shanghai Science and Technology Committee Foundation, 10411966500

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摘要：

目的 分析子前期（PE）患者胎盘组织中C型凝集素样受体家族中DC-SIGN和甘露糖受体（MR）以及Toll样受体（TLR）家族中TLR-2和TLR-4的表达情况。方法 采集PE重度患者（PE组，n=20）及正常妊娠妇女（NP组，n=20）的新鲜胎盘标本；采用免疫组织化学技术对胎盘组织中DC-SIGN、MR、TLR-2和TLR-4的表达进行定位分析；采用Western blotting法检测胎盘组织中的DC-SIGN、MR、TLR-2和TLR-4的蛋白表达。结果 两组胎盘组织中均有DC-SIGN、MR、TLR-2和TLR-4的表达，主要位于胎盘合体滋养层细胞，在细胞滋养层细胞中亦有少量表达。PE组与NP组比较，胎盘组织TLR-4阳性细胞表达率明显升高（P<0.01）；DC-SIGN、MR阳性细胞表达率明显降低（P<0.01，P<0.05），TLR-2阳性细胞表达率的差异无统计学意义（P>0.05）。结论 相对于正常晚期妊娠妇女，PE患者胎盘组织中C型凝集素受体DC-SIGN和MR表达减少，TLR-4过度表达；两种受体家族活化平衡失调可能是PE发病机制中的关键环节之一。

关键词: 子前期, 胎盘, C型凝集素样受体, Toll样受体

Abstract:

Objective To investigate the expression of C-typed lectin receptors of dendritic cells specific intercellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN) and mannose receptor (MR) and Toll-like receptors (TLR) of TLR-2 and TLR-4 in placenta tissues in patients with pre-eclampsia (PE). Methods The placenta tissues were obtained from patients with severe pre-eclampsia (PE group, n=20) and normal pregnant women (NP group, n=20). The expression of DC-SIGN, MR, TLR-2 and TLR-4 in placenta tissues was localized by immunohistochemistry, and the expression of DC-SIGN, MR, TLR-2 and TLR-4 protein in placenta tissues was detected by Western blotting. Results There was expression of DC-SIGN, MR, TLR-2 and TLR-4 in syncytiotrophoblasts and cytotrophoblasts of placenta tissues in both groups, and the majority was in syncytiotrophoblasts. The expression of TLR-4 in placenta tissues in PE group was significantly higher than that in NP group （P<0.01）, the expression of DC-SIGN and MR in placenta tissues in PE group was significantly lower than that in PE group (P<0.01, P<0.05), and there was no significant difference in the expression of TLR-2 in placenta tissues between two groups (P>0.05). Conclusion Compared with normal pregnant women, the expression of C-typed lectin receptors of DC-SIGN and MR is lower, while the expression of TLR-4 is abnormally higher in placenta tissues in patients with PE. The imbalance between these two receptors may play an important role in the pathogenesis of PE.

Key words: pre-eclampsia, placenta, C-type lectin receptors, Toll-like receptors