上海交通大学学报（医学版）

›› 2012, Vol. 32 ›› Issue (8): 1005-.doi: 10.3969/j.issn.1674-8115.2012.08.010

• 专题报道（不孕不育症及辅助生殖技术） • 上一篇    下一篇

体外受精周期中血清和卵泡液活性氧及总抗氧化能力的研究

牛志宏, 吴丹丹, 林 楠, 孙贻娟, 陆小溦, 冯 云   

  1. 上海交通大学 医学院附属瑞金医院生殖医学中心, 上海 200025
  • 出版日期:2012-08-28 发布日期:2012-08-29
  • 通讯作者: 冯 云, 电子信箱: artruijin@yahoo.com.cn。
  • 作者简介:牛志宏（1975—）, 女, 主治医师, 博士；电子信箱: kangniu@sina.com。

Study on reactive oxygen species and total antioxidant capacity of serum and follicluar fluid in in vitro fertilization cycles

NIU Zhi-hong, WU Dan-dan, LIN Nan, SUN Yi-juan, LU Xiao-wei, FENG Yun   

  1. Reproductive Medical Center, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China
  • Online:2012-08-28 Published:2012-08-29

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摘要：

目的 探讨体外受精（IVF）周期中血清和卵泡液活性氧（ROS）及总抗氧化能力（TAC）的变化特征。方法 选择进行IVF助孕的患者共245个周期，分别收集卵泡刺激前基础状态血清和取卵日血清及排卵期卵泡液，检测ROS和TAC，并分析其与相关临床特征之间的相关性。结果 基础状态血清ROS值平均为（59.8±54.6）cps，取卵日血清ROS值平均为（77.5±62.3）cps，差异具有统计学意义（P＜0.05）；但TAC差异无统计学意义（P＞0.05）。卵泡液ROS与取卵日血清ROS、卵泡液TAC与基础状态血清TAC及取卵日TAC均呈正相关（P＜0.05）。卵泡液ROS和TAC水平与患者年龄、 雌二醇（E2）峰值、获卵数、峰值E2/获卵数及受精率等均无相关性，仅ROS与患者体质量指数（BMI）呈正相关（r=0.29，P=0.04）。BMI＜19 kg/m2组与BMI 为23～25 kg/m2组及BMI＞25 kg/m2组的患者相比，卵泡液ROS显著降低（P＜0.05）。结论 排卵期血清ROS较早卵泡期升高，卵泡液ROS与BMI呈正相关。

关键词: 活性氧, 总抗氧化能力, 卵泡液, 体外受精

Abstract:

Objective To investigate the characteristics of reactive oxygen species (ROS) and total antioxidant capacity (TAC) of serum and follicular fluid in in vitro fertilization (IVF) cycles. Methods Patients (245 cycles) undergoing IVF were enrolled. Blood samples of early follicle phase and ovum retrieval day and follicular fluid of ovulation phase were collected, ROS and TAC were examined, and their relationship with clinical characteristics was analysed. Results Serum ROS on ovum retrieval day was significantly higher than that in early follicle phase [(77.5±62.3) cps vs (59.8±54.6) cps, P＜0.05], while there was no significant difference in TAC (P＞0.05). There was positive correlation between follicular ROS and serum ROS on ovum retrieval day, and between follicular TAC and serum TAC of early follicle phase and ovum retrieval day (P＜0.05). There was no correlation of follicular ROS and TAC with age, peak estradiol (E2), oocyte number, peak E2/oocyte number and fertilization rate. However, follicular ROS was positively related to body mass index (BMI)(r=0.29，P=0.04). Follicular ROS in patients with BMI＜19 kg/m2 was significantly lower than those in patients with BMI 23-25 kg/m2 and those with BMI＞25 kg/m2 (P＜0.05). Conclusion Serum ROS in ovulation phase is higher than that in early follicular phase, follicular ROS has a positive correlation with BMI.

Key words: reactive oxygen species, total antioxidant capacity, follicular fluid, in vitro fertilization