上海交通大学学报（医学版）

›› 2013, Vol. 33 ›› Issue (2): 184-.doi: 10.3969/j.issn.1674-8115.2013.02.012

• 论著（临床研究） • 上一篇    下一篇

卵泡液和血清EG-VEGF、VEGF和TGF-β1水平与IVF妇女卵巢反应的关系及其对妊娠结局的预测价值

高敏芝1,2, 孙兆贵3, 赵晓明1, 张慧琴2,3   

  1. 1.上海交通大学 医学院附属仁济医院生殖医学科, 上海 200001； 2.复旦大学 上海医学院, 上海 200032； 3.国家人口和计划生育委员会计划生育药具重点实验室 |上海市计划生育科学研究所, 上海 200032
  • 出版日期:2013-02-28 发布日期:2013-03-07
  • 通讯作者: 赵晓明, 电子信箱: zhao_xiao_ming@hotmail.com。
  • 作者简介:高敏芝（1974—）, 女, 副主任医师, 博士； 电子信箱: 328gao@sina.com。
  • 基金资助:

    上海交通大学医工交叉研究基金（YG2011MS42）

Association of follicular and serum EG-VEGF, VEGF and TGF-β1 levels with ovarian response in women with IVF and their predictive values for pregnancy outcome

GAO Min-zhi1,2, SUN Zhao-gui3, ZHAO Xiao-ming1, ZHANG Hui-qin2,3   

  1. 1.Department of Reproductive Medicine, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200001, China； 2.Shanghai Medical College, Fudan University, Shanghai 200032, China； 3.Key Laboratory of Contraceptive Drugs &|Devices of NPFPC, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China
  • Online:2013-02-28 Published:2013-03-07
  • Supported by:

    Shanghai Jiaotong University Foundation, YG2011MS42

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摘要：

目的 探讨卵泡液和血清中内分泌腺来源的血管内皮生长因子(EG-VEGF）、血管内皮生长因子（VEGF）和转化生长因子β1（TGF-β1）水平与体外受精（IVF）妇女卵巢反应的关系及其对妊娠结局的预测价值。方法 选择60例因输卵管和（或）男性因素而接受体外受精-胚胎移植(IVF-ET)治疗的不孕症患者作为研究对象。ELISA法检测取卵当日卵泡液中和取卵后第2天血清中EG-VEGF、VEGF和TGF-β1的水平，分析其与卵巢反应和IVF妊娠结局的关系。结果 卵泡液EG-VEGF、血清EG-VEGF、卵泡液VEGF浓度均与控制性超排卵周期中人绒毛膜促性腺激素(hCG)注射日血清雌二醇（E2）浓度呈显著负相关（r=－0.622, P<0.01; r=－0.511, P<0.01; r=－0.427, P<0.01），卵泡液EG-VEGF与卵泡液VEGF浓度呈显著正相关（r=0.354, P<0.01）。60例不孕症患者中，妊娠31例，未妊娠29例。与非妊娠组比较，妊娠组hCG注射日血清E2浓度较低（P<0.05），而血清EG-VEGF浓度较高（P<0.01）；而两组间卵泡液和血清VEGF、TGF-β1的浓度比较，差异均无统计学意义（P>0.05）。结论 卵泡液和血清EG-VEGF及卵泡液VEGF水平均与卵巢反应呈显著负相关。黄体早期血清EG-VEGF水平与IVF妊娠结局相关，可能具有预测IVF妊娠结局的潜在价值。

关键词: 内分泌腺来源的血管内皮生长因子, 血管内皮生长因子, 转化生长因子β1, 体外受精—胚胎移植, 控制性超排卵, 妊娠结局

Abstract:

Objective To investigate the association of follicular and serum levels of endocrine gland-derived vascular endothelial growth factor (EG-VEGF), vascular endothelial growth factor (VEGF) and transforming growth factor-β1 (TGF-β1) with ovarian response in women with in vitro fertilization (IVF), and explore their predictive values for pregnancy outcome. Methods Sixty infertile women undergoing in vitro fertilization and embryo transfer (IVF-ET) were selected, the follicular levels of EG-VEGF, VEGF and TGF-β1 during oocyte retrieval and the serum levels of EG-VEGF, VEGF and TGF-β1 on day 2 after oocyte retrieval were measured, and their association with ovarian response and pregnancy outcome of IVF were analysed.ResultsThe follicular level of EG-VEGF, serum level of EG-VEGF and follicular level of VEGF were negatively correlated with serum level of estradiol (E2) on the day of human chorionic gonadotropin (hCG) injection during controlled ovarian hyperstimulation (r=－0.622, P<0.01; r=－0.511, P<0.01; r=－0.427, P<0.01). The follicular level of EG-VEGF was positively correlated with follicular level of VEGF (r=0.354, P<0.01). Among the 60 patients, 31 were pregnant, while the other 29 were not. The serum level of E2 on the day of hCG injection in pregnancy group was significantly lower than that in non-pregancy group (P<0.05), and the serum level of EG-VEGF in pregnancy group was significantly higher than that in non-pregancy group (P<0.01), while there was no significant difference in follicular and serum levels of VEGF and TGF-β1 between two groups (P>0.05). Conclusion Follicular and serum levels of EG-VEGF and follicular level of VEGF are negatively correlated with ovarian response. Serum level of EG-VEGF in the early luteal phase is associated with pregnancy outcome of IVF, which may potentially predict the pregnancy outcome of IVF.

Key words: endocrine gland-derived vascular endothelial growth factor, vascular endothelial growth factor, transforming growth factor-β1, in vitro fertilization and embryo transfer, controlled ovarian hyperstimulation, pregnancy outcome