上海交通大学学报（医学版）

›› 2013, Vol. 33 ›› Issue (3): 294-.doi: 10.3969/j.issn.1674-8115.2013.03.008

• 论著（临床研究） • 上一篇    下一篇

芳香烃受体与原因不明自然流产关系的初步研究

范鸿杰1, 陆继红2, 杨宾烈3, 赵爱民1   

  1. 1.上海交通大学 医学院附属仁济医院妇产科, 上海 200127； 2.上海浦东新区公利医院妇产科, 上海 200135； 3.上海市浦东新区人民医院妇产科, 上海 201200
  • 出版日期:2013-03-28 发布日期:2013-03-29
  • 通讯作者: 赵爱民, 电子信箱: zam0526@yahoo.com.cn。
  • 作者简介:范鸿杰(1987—), 女, 硕士生； 电子信箱: fan083@163.com。
  • 基金资助:

    上海市浦东新区卫生局卫生科技项目重点协作项目(PW2011D-3)

Preliminary study of relationship between aryl hydrocarbon receptor and unexplained spontaneous abortion

FAN Hong-jie1, LU Ji-hong2, YANG Bin-lie3, ZHAO Ai-min1   

  1. 1.Department of Obstetrics and Gynecology, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200127, China； 2.Department of Obstetrics and Gynecology, Shanghai Pudong New Area Gongli Hospital, Shanghai 200135, China； 3.Department of Obstetrics and Gynecology, Shanghai Pudong New Area People´s Hospital, Shanghai 201200, China
  • Online:2013-03-28 Published:2013-03-29
  • Supported by:

    Shanghai Pudong New Area Health Bureau Foundation, PW2011D-3

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摘要：

目的 初步探讨早孕蜕膜和绒毛组织中芳香烃受体（AhR）的表达及其与原因不明自然流产的关系。方法 采用Real-Time PCR和Western blotting技术检测34例原因不明自然流产患者(流产组)蜕膜和绒毛组织中AhR的mRNA和蛋白表达水平；以38例正常孕早期妇女作为对照(对照组)。结果 对照组和流产组绒毛组织AhR mRNA和蛋白表达水平均高于蜕膜组织(P<0.01，P<0.05)；流产组蜕膜组织中AhR mRNA和蛋白表达均高于对照组(P<0.01，P<0.05)；流产组绒毛组织中AhR mRNA和蛋白表达均高于对照组(P<0.01，P<0.05)。结论 早孕绒毛组织中AhR mRNA和蛋白的表达水平均明显高于蜕膜组织，其表达上调可能与原因不明自然流产的发生和发展有关。

关键词: 芳香烃受体, 原因不明自然流产, 蜕膜, 绒毛

Abstract:

Objective To investigate the expression of aryl hydrocarbon receptor (AhR) in decidua and villus, and explore its relationship with unexplained spontaneous abortion. Methods Real-time PCR and Western blotting were employed to detect the expression of AhR mRNA and protein in decidua and villus of 34 patients with unexplained spontaneous abortion (abortion group), and 38 women of normal early pregnancy were served as control group. Results The expression of AhR mRNA and protein in villus was significantly higher than that in decidua in two groups (P＜0.01, P＜0.05). The expression of AhR mRNA and protein in decidua in abortion group was significantly higher than that in control group (P＜0.01, P＜0.05). The expression of AhR mRNA and protein in villus in abortion group was significantly higher than that in control group (P＜0.01, P＜0.05). Conclusion The expression of AhR mRNA and protein in villus is significantly higher than that in decidua, and the upregulation of expression may be associated with the pathogenesis of unexplained spontaneous abortion.

Key words: aryl hydrocarbon receptor, unexplained spontaneous abortion, decidua, villus