›› 2012, Vol. 32 ›› Issue (12): 1567-.doi: 10.3969/j.issn.1674-8115.2012.12.011

• Original article (Basic research) • Previous Articles     Next Articles

Effects of injured meninges fibroblasts on viability and expression of glial fibrillary acidic protein of astrocytes

LI Yi1, CHEN Xue1, CHEN Ying1, LIN Wei-wei1, LIU Yong-hua1, ZHU Jun-jie1, FEI Xiang1, LIU Yu-xi1, WANG Xiao-dong2   

  1. 1.Department of Histology and Embryology, Medical College, Nantong University, Nantong 226001, China；2.Department of Neurosurgery, Affiliated Hospital of Nantong University, Nantong 226001, China
  • Online:2012-12-28 Published:2012-12-31
  • Supported by:

    National Natural Science Foundation of China, 30870643；Jiangsu College Natural Science Research Foundation, 11KJB310007；Jiangsu College Excellent Discipline Construction Foundation；Nantong University Foundation, 10Z055

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Abstract:

Objective To investigate the in vitro effects of injured meninges fibroblasts on viability and expression of glial fibrillary acidic protein (GFAP) of astrocytes. Methods Astrocytes from cerebra cortices and fibroblasts from meninges of SD rats were cultured respectively, and were identified by GFAP and fibronectin(FN) immunofluorescence staining. According to the terms of scratch injury of fibroblasts, astrocytes co-cultured with fibroblasts derived conditional medium were divided into normal control group (group A), 4-h injury group (group B), 24-h injury group (group C) and 72-h injury group (group D). The viability of astrocytes was determined by CCK8 assay, and the expression of GFAP of astrocytes was detected by GFAP immunofluorescence staining. At the same time, contact co-culture was conducted in injured fibroblasts and astrocytes for 72 h. The astrocytes were divided into short distance group (group J) and long distance group (group Y) according to the distance from fibroblasts, and the expression of GFAP was detected with FN and GFAP double immunofluorescence cytochemical staining. Results The viability of astrocytes in group B was significantly lower than that in group A, group C and group D (P<0.05). The expression of GFAP of astrocytes in group C was significantly higher than that in group B and group D (P<0.05). The expression of GFAP of astrocytes in group J was significantly higher than that in group Y (P<0.05). Conclusion Injured meninges fibroblasts are able to trigger reactive astrogliosis.

Key words: fibroblasts, astrocytes, viability, glial fibrillary acidic protein