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Protective effects of different doses of lanthanum chloride on inflammatory reaction of RAW264.7 cells induced by alumina ceramic particle

NIE Tao, DAI Min, JIANG Chuan, LIU Xiang   

  1. Department of Orthopedics, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China
  • Online:2014-08-28 Published:2014-09-02

Abstract:

Objective To observe the effects of different doses of lanthanum chloride (LaCl3) on inflammatory reaction of rat macrophage RAW264.7 induced by the alumina (Al2O3) ceramic particle and to provide the theoretical basis for studying the effects of LaCl3 on aseptic loosening induced by the Al2O3 ceramic particle. Methods RAW264.7 cells were cultured in vitro and randomly divided into eight groups according to different culture solutions, i.e. the blank control group (no LaCL3 and Al2O3 in culture medium), Al2O3 group (1 mg/mL Al2O3), LaCl3+Al2O3 groups (Al2O3 of 1 mg/mL was added, than LaCl3 of 2.5, 10, and 100 μmol/L were added, respectively), and LaCl3 groups (LaCl3 of 2.5, 10, and 100 μmol/L). The expression of inflammatory factors IL-1β, TNF-α, and NF-κb were detected by the ELISA, RT-PCR, and Western blotting, respectively. Results The results of ELISA showed that compared to the blank control group, the expressions of IL-1β and TNF-α of the Al2O3 group and LaCl3 group (LaCl3 of 100 μmol/L) were significantly higher and the differences were statistically significant (P<0.05). Compared to the Al2O3 group, the expressions of IL-1β and TNF-α of the LaCl3+Al2O3 groups (LaCl3 of 2.5 and 10 μmol/L) were significantly lower and the differences were statistically significant (P<0.05). The expression of IL-1β and TNF-α of the LaCl3 group (LaCl3 of 2.5 and 10 μmol/L) were significantly lower than those of the LaCl3 group (LaCl3 of 100 μmol/L) and the differences were statistically significant (P<0.05). The results of the RT-PCR and Western blotting showed that the variation trends of expressions of IL-1β, TNF-α, NF-κB mRNA and NF-κB protein were consistent with those detected by the ELISA. Conclusion LaCl3 of 10 μmol/L can inhibit the excretion of IL-1β and TNF-α of macrophage to some extent. The inhibition of inflammation of LaCl3 may be relevant to the inhibition of NF-κB signal transduction pathway.

Key words: interleukin-1β, tumor necrosis factor-α, nuclear factor-κB, lanthanum chloride, alumina ceramic particles