›› 2017, Vol. 37 ›› Issue (6): 732-.doi: 10.3969/j.issn.1674-8115.2017.06.004

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Vitamin E could reverse effects of bisphenol A on steroidogenesis in rat ovary granulosa cells

CHEN Wei-wei, SUN Wei-jie, WANG Lei, JIAO Xian-ting, YU Xiao-dan   

  1. MOE-Shanghai Key Lab of Children’s Environmental Health, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China
  • Online:2017-06-28 Published:2017-07-05
  • Supported by:

    National Key Basic Research Program of China, “973” Program,  2014CB943300; Shanghai Municipal Education Commission—Gaofeng Clinical Medicine Grant Support, 20152220


 Objective · To investigate whether vitamin E could reverse the disruptive effects of bisphenol A (BPA) on steroidogenesis and to explore the optimal vitamin E concentration.  Methods · Rat primary granulosa cells were extracted and exposed to BPA (0, 0.01, 0.1,1,10,100 μmol/L). After 48 h of incubation, culture medium was collected and estradiol (E2) and progesterone (P4) were measured using ELISA kits. Then, granulosa cells were incubated with 5 μmol/L(average concentration in follicular fluid) or 25 μmol/L (high concentration in follicular fluid) vitamin E (α-tocopherol) or vitamin E (5 μmol/ L or 25 μmol/L) plus BPA (10 μmol/L or 100 μmol/L) for 48 h, E2 and P4 were measured.  Results · BPA at 10 μmol/L and 100 μmol/L suppressed E2 and P4 production in a dose-dependent manner (P<0.05). Vitamin E at 25 μmol/L significantly increased E2 and P4 levels by (44.89±15.18) % and (43.33±8.82) % (P<0.05), respectively. Coincubation of the granulosa cells with BPA and vitamin E (5 μmol/L or 25 μmol/L) restored the productions of E2 and P4, which were not significantly different from the control group (P>0.05).  Conclusion · Vitamin E (5 μmol/L/ 25 μmol/L) could reverse BPA-induced reduction in steroid hormone production in rat ovary granulosa cells, and the antagonistic effect of 25 μmol/L vitamin E was more obvious than that of 5 μmol/L vitamin E.

Key words: bisphenol A , vitamin E, ovary granulosa cell, estradiol, progesterone