›› 2019, Vol. 39 ›› Issue (7): 698-.doi: 10.3969/j.issn.1674-8115.2019.07.003

• Original article (Basic research) • Previous Articles     Next Articles

Bioinformatics analysis of differentially expressed genes in ischemic cardiomyopathy

ZHANG Peng1, CHANG Zheng-yan2, YANG Lei1, XUE Song1, LIAN Feng1   

  1. 1. Department of Cardiovascular Surgery, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200127; 2. Department of Pathology, Shanghai Tenth Peoples Hospital of Tongji University, Shanghai 200072
  • Online:2019-07-28 Published:2019-08-26
  • Supported by:
    National Natural Science Foundation of China, 81873461; Shanghai Jiao Tong University Medicine-Engineering Cross Research Foundation,YG2015MS51; Specialized Disease Clinical“Five New”Transformation Project, 16CR3086B

Abstract: Objective · To screen differentially expressed genes in ischemic cardiomyopathy (ICM)bioinformatics analysis and construct the regulatory network of competing endogenous RNA (ceRNA). Methods · Data sets were downloaded Gene Expression Omnibus (GEO) database to screen the differentially expressed mRNAs and lncRNAs between normal samples and ICM ones. Then, GO (Gene Ontology) analysis and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis were performed. The differentially expressed mRNAs and lncRNAs were analyzed to predict related miRNAsbioinformatics methods, and then to construct ceRNA regulatory network. Results · GO analysis and KEGG pathways analysis results showed that the differentially expressed genes were enriched in the pathways such as metabolic pathway, oxidative phosphorylation, and extracellular matrix receptor interaction, and they were closely related to the functions such as endoplasmic reticulum stress, fibrosis, collagen catabolic process, and inflammatory response. A ceRNA regulatory network containing 26 mRNAs, 2 lncRNAs and 15 miRNAs was constructed. Conclusion · The bioinformatics method can be used to analyze the differentially expressed genes of ICM, and the regulatory network of ceRNA of ICM has been successfully constructed.

Key words: ischemic cardiomyopathy (ICM), differentially expressed gene, long non-coding RNA (lncRNA), bioinformatics analysis, competing endogenous RNA (ceRNA)

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